Citation: GAI Pan-Pan, KONG Xin-Ke, ZHAO Shi-Fan, LI Feng. Self-Powered Platform for Heparin Ultrasensitive Detection Based on Anion-Exchange Mechanism[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(9): 1141-1148. doi: 10.19756/j.issn.0253-3820.201249 shu

Self-Powered Platform for Heparin Ultrasensitive Detection Based on Anion-Exchange Mechanism

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China

Received Date: 1 May 2020
Revised Date: 1 June 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21605092, 21775082)

Biofuel cell-based self-powered biosensor features with the advantages such as no extra power supplied, simple instrumentation, miniaturization and excellent anti-interference capability because the signal of which is directly proportional to the performance of the biofuel cell. However, the performance of self-powered biosensors needs to be improved because the biocatalysts of biofuel cells are easily deactivated. Herein, we developed a biofuel cell-based self-powered biosensor for the ultrasensitive heparin (Hep) detection via integration of the anion-exchange mechanism and molecular imprinting technology. In this case, molecular imprinting technology-based nanoporous gold acted as the bioanode and the potassium ferricyanide/polyimidazolium/reduced graphene oxide (Fe(CN)₆^3-/Pim/rGO) complex played the role of the biocathode. In the absence of Hep, the biofuel cell-based self-powered biosensor could achieve a high open-circuit voltage and power density output. In the presence of Hep, Fe(CN)₆^3- broke away from the Pim due to the stronger reaction between the Hep and Pim, which resulted in the electron acceptor decreased on the surface of the biocathode and further reduced the signal. Finally, the open-circuit voltage and power density output were reduced and the ultrasensitive Hep detection was realized via the change of the performance of biofuel cell. The detection limit was 0.01 pmol/L (S/N=3), being superior to the reported methods.
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