Citation: YANG Rui, KANG Tian-Fang, LU Li-Ping, YE Qing. Highly Sensitive Detection of Pb²⁺ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(2): 309-317. doi: 10.19756/j.issn.0253-3820.201231 shu

Highly Sensitive Detection of Pb²⁺ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry

Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Corresponding author: KANG Tian-Fang, kangtf@bjut.edu.cn
Received Date: 25 April 2020
Revised Date: 18 November 2020

Fund Project: Supported by the Scientific Technological Project Foundation of Beijing Municipal Scientific Technological Commission (No.Z171100002217002) and the Doctoral Fund of China Education Ministry (No.20131103110011).

The composite of gold nanoparticles-graphene oxide(AuNPs-GO) was prepared and characterized by transmission electron microscope (TEM) and ultraviolet-visible(UV-vis) spectrophotometry, respectively. The composite was modified on the surface of a glassy carbon electrode (GCE). The mercapto-modified and guanine-rich DNA strand (T30695) was self-assembled on to gold nanoparticles on the surface of the electrode via Au-S bond. T30695 could combine with lead ions (Pb²⁺) to form a stable parallel G-quadruplex. The G-quadruplex further combined with hemin to form DNAzyme with peroxidase activity. On the basis of this, a DNAzyme electrochemical sensor for Pb²⁺ detection was prepared. The DNAzyme could catalyze the oxidation reaction of hydrogen peroxidetowardshydroquinone (HQ) to achieve the amplification of electrochemical signal and highly sensitive detection of Pb²⁺. Under the optimum conditions, the peak current of square-wave voltammetry (SWV) was linearly dependent on the logarithm of Pb²⁺ concentration ranging from 5.0×10^-9 mol/L to 1.0×10^-6 mol/L with a detection limit of 1.0×10^-9 mol/L(S/N=3). The recovery of Pb²⁺ in real water sample was 96%-105%. The sensor possessed good reproducibility, stability and selectivity to Pb²⁺.
- Square-wave voltammetry,
- Sensor,
- DNAzyme,
- Lead ion
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Highly Sensitive Detection of Pb²⁺ Based on DNAzyme/AuNPs-GO Sensor by Square-Wave Voltammetry