Citation: YUAN Min, WANG Meng-Xue, Zheng Yu-Zhu, CAO Hui, XU Fei, YE Tai, YU Jin-Song. Aptamer/Gold Nanoparticles-based Fluorometric and Colorimetric Dual-Mode Detection of Arsenite[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(1): 76-84. doi: 10.19756/j.issn.0253-3820.201180 shu

Aptamer/Gold Nanoparticles-based Fluorometric and Colorimetric Dual-Mode Detection of Arsenite

YUAN Min ¹ ,
WANG Meng-Xue ¹ ,
Zheng Yu-Zhu ¹ ,
CAO Hui ¹ ,
XU Fei ^1,2,, ,
YE Tai ¹ ,
YU Jin-Song ¹

1.
School of Medical Instruments and Food Engineering, Shanghai Engineering Research Center for Food Rapid Detection, University of Shanghai for Science and Technology, Shanghai 200093, China;
2.
Institute of Food Quality and Safety, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: XU Fei, xufei8135@126.com
Received Date: 5 April 2020
Revised Date: 10 September 2020

Fund Project: Supported by the National Natural Science Foundation of China (Nos. 31671934, 61501295), the Ministry of Science and Technology of China (No. 2017YFC1600603), and the Project of Shanghai Committee of Science and Technology (Nos.18391901200, 17391901500).

On the basis of fluorescence resonance energy transfer of gold nanoparticles (AuNPs) to fluorophore and the unique optical effect of AuNPs and in combination with arsenite aptamer, a dual mode of fluorescent and colorimetric detection method for inorganic arsenite was developed. In this method, the unmodified AuNPs were used as the carrier, and the aptamer of arsenic modified by FAM (FAM-Apt) as the probe was adsorbed on the surface of AuNPs. Due to the fluorescence resonance energy transfer between FAM-Apt and AuNPs, the fluorescence of FAM-Apt could be quenched by AuNPs. When arsenite was present, it combined with FAM-Apt, so that it was released from the surface of AuNPs, which induced the fluorescence recovery. And the gold nanoparticles without the protection of aptamer FAM-Apt were easily induced to aggregate, and the color changed from red into blue in the presence of salt. Thus a dual-mode detectior of fluorescence and colorimetry was developed for As(Ⅲ) detection. Both the fluorescence and colorimetric adsorption showed a good linear relationship with arsenite concentration. The fluorescence method showed a detection range of 5-800 μmol/L with a detection limit of 3.64 μmol/L (3σ), while the colorimetric method showed a detection range of 5-100 μmol/L with a detection limit of 3.42 μmol/L (3σ). And the dual-mode combined with fluorescence and colorimetry showed a linear detection range of 5-1000 μmol/L with a detection limit of 1.55 μmol/L. This method was simple, fast and easy to operate. The dual-mode method illustrated a lower detection limit and a wider detection range. The two detection modes could verify each other, and were successfully used in detection of As(Ⅲ) in fruit and vegetables. Moreover, the method could provide a general strategy for detecting other small biomolecules, metal ions and proteins.
- Arsenite (Ⅲ),
- Aptamer,
- Gold nanoparticles,
- Fluorescence,
- Colorimetric,
- Dual-mode detection
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