Citation: XIE Han-Zhao, YANG Bin, LI Jian-Ping. A Molecularly Imprinted Electrochemical Luminescence Sensor for Detection of Gibberellin Based on Energy Transfer[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(12): 1633-1641. doi: 10.19756/j.issn.0253-3820.201179 shu

A Molecularly Imprinted Electrochemical Luminescence Sensor for Detection of Gibberellin Based on Energy Transfer

College of Chemical and Bioengineering, Guilin University of Technology, Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, Guilin 541004, China

Corresponding author: LI Jian-Ping, likianping@263.net
Received Date: 5 April 2020
Revised Date: 8 August 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21765006), the Guangxi Natural Science Foundation of China (No. 2018GXNSFAA138145) and Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, China.

A molecularly imprinted sensor was fabricated, and a method based on enhancing electrochemiluminescence (ECL) signal of Ru(bpy)₃Cl₂ using resonance energy transfer (RET) was established for ultra-sensitive determination of gibberellin (GA3). Au-doped g-C₃N₄ (g-C₃N₄/Au) was prepared on the surface of glassy carbon electrode, and then o-phenylenediamine was electropolymerized to achieve a molecularly imprinted polymer (MIP) film. The film was then eluted by methanol-acetic acid to obtain molecularly imprinted cavities for specifically recognizing GA3. Ru(bpy)₃²⁺ was used as probe and g-C₃N₄/Au was used as energy donor. The RET between Ru(bpy)₃²⁺ and g-C₃N₄/Au was used to enhance ECL intensity of Ru(bpy)₃²⁺. In addition, ECL-RET was combined with molecular imprinting technology to improve selectivity of the sensor. With the increase of the concentration of GA3, the molecularly imprinted cavities recombined with GA3, which made the ECL intensity gradually decreased. Under the optimized conditions, there was a linear relationship between ECL intensities and GA3 concentrations in the range from 4.00×10^-14 mol/L to 7.00×10^-11 mol/L, and the detection limit was 1.64×10^-14 mol/L (3S/K). The sensor was successfully applied to the detection of GA3 in beer, and the recoveries were 95.7%-103.7%.
- Molecularly imprinting,
- Gibberellins,
- Electrochemical luminescence,
- Resonance energy transfer,
- g-C₃N₄/Au
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