Citation: ZHU Chao, ZHAO Xin-Ying, YANG Ge, QU Feng. Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 583-589. doi: 10.19756/j.issn.0253-3820.201173 shu

Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers

ZHU Chao ^1, ,
ZHAO Xin-Ying ² ,
YANG Ge ¹ ,
QU Feng ¹

1.
School of Life Science, Beijing Institute of Technology, Key Laboratory of Molecular Medicine and Biological Diagnosis, Ministry of Industry and Information Technology, Beijing 100081, China;
2.
Beijing Center for Physical and Chemical Analysis, Beijing 100089, China

Received Date: 31 March 2020
Revised Date: 16 April 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 20875009, 21175011, 21375008, 21675012, 21874010, 21827810) and the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology (2020-2022).

Systematic evolution of ligands by exponential enrichment (SELEX) is a universal technology for aptamers’ screening. Accurate and efficient screening methods and strategy design are the key to successful screening of aptamers. This article briefly summarizes our works of capillary electrophoresis involving in the efficient screening of aptamers since 2007. The review also introduces the employment of capillary electrophoresis in pre-screening, screening and post-screening SELEX process, classification and screening strategies against different protein targets, multiple capillary electrophoresis screening modes, and aptamers screening for multi-scale targets. Finally, combined with the current research progress of aptamer screening in the world, some remaining issues are proposed.
- Capillary Electrophoresis,
- Screening strategy for aptamers,
- Multiple modes screening,
- Multi-scale targets,
- Review
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