Citation: FU Yan-Feng, YANG Yu, ZHANG Qing-Rong, CAI Ming-Jun, XU Hai-Jiao, YANG Guo-Cheng, SHAN Yu-Ping. Atomic Force Microscopy-based Nanoindentation Technique for Evaluation of Efficacy of Nano-drugs[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(9): 1219-1227. doi: 10.19756/j.issn.0253-3820.201171 shu

Atomic Force Microscopy-based Nanoindentation Technique for Evaluation of Efficacy of Nano-drugs

1.
School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 31 March 2020
Revised Date: 26 May 2020

Fund Project: This work was supported by the National Natural Science Foundation of China(Nos. 21773017, 21673023).

Nano-drugs with dual advantages of targeted drug delivery and controllable release have become a new research hotspot of anti-tumor drugs. In the application of nano-drugs, the evaluation of drug efficacy is a very important issue. Herein, nanoindentation based on atomic force microscopy was used to analyze the changes in the mechanical properties of cells (Young's modulus and viscoelasticity, etc.) co-incubated with nano-drugs to measure the drug efficacy. The effect of doxorubicin (DOX) and camptothecin (CPT) in liposomes on cervical cancer (HeLa) cells was more significant than that of free DOX. Furthermore, the difference in the efficacy of liposomes mixed with DOX and CPT at different molar ratios (4:1 and 1:4) on HeLa cells were detected, and it was found that the synergistic effect of the two drugs enhanced the efficacy at 4:1 molar ratio. Finally, the viscoelasticity of HeLa cells co-incubated with nano-drugs was evaluated, and it was found that the viscoelasticity of the cells decreased with the extension of the co-incubation time of nano-drugs. This study provided a simple and effective method for screening nano-drugs.
- Atomic force microscopy,
- Nanoindentation,
- Liposomes,
- Nano-drug efficacy,
- Cell mechanics
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