Citation: ZHANG Hua, LI Qun, LIU Gui-Feng. Gold Nanorods-based Immunochromatographic Strip for Rapid and Quantitative Detection of Prostate Specific Antigen[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(8): 1018-1024. doi: 10.19756/j.issn.0253-3820.201130 shu

Gold Nanorods-based Immunochromatographic Strip for Rapid and Quantitative Detection of Prostate Specific Antigen

ZHANG Hua ^2, ,
LI Qun ¹ ,
LIU Gui-Feng ¹

1.
Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 14 March 2020
Revised Date: 20 May 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No.61901438).

A gold nanorod (GNR)-based immunochromatographic strip was developed for rapid and quantitative detection of prostate specific antigen (PSA). GNR was used as the labeled probe and coated with sodium polyacrylate. Then the anti-PSA monoclonal antibody (dAb) was immobilized on the surface of GNR through covalent attachment to form the GNR-dAb conjugates. The mouse anti-PSA monoclonal antibody and the goat anti-mouse IgG were sprayed onto the nitrocellulose membrane as test line and control line, respectively. The resultant GNR-dAb conjugates were introduced to construct the immunochromatographic strip for the quantitative detection of PSA by a sandwich method. The proposed GNR-based immunochromatographic strip exhibited good specificity, high stability and high sensitivity. The experimental results indicated that GNR-based immunochromatographic strip showed a good linear range from 0.1 ng/mL to 50 ng/mL with a limit of detection of 0.1 ng/mL, and the relative standard deviation was less than 10%. The established assay could be successfully applied for PSA detection in serum, which was beneficial for early diagnosis, treatment and prognosis of prostate cancer.
- Gold nanorod,
- Immunochromatography,
- Prostate specific antigen,
- Quantitative detection
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
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