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Citation: XIE Xiao-Dong, YIN Min, LI Qian, CHEN Nan. Imaging of Cell Migration Mediated Exocytosis with Gold Nanoprobes[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(7): 847-854. doi: 10.19756/j.issn.0253-3820.201109 shu

Imaging of Cell Migration Mediated Exocytosis with Gold Nanoprobes

XIE Xiao-Dong ^1,2, ,
YIN Min ³ ,
LI Qian ⁴ ,
CHEN Nan ³

1.
Division of Physical Biology, Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
Department of Chemistry, Shanghai Normal University, Shanghai 200234, China;
4.
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

Received Date: 4 March 2020
Revised Date: 29 March 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 31771102, 21974089, 91953016).

Insight into the exocytosis process of diagnostical nanomaterials is critical for their clinical transformation. However, it is unclear whether nanomaterials will be eliminated during cell migration. Herein, gold nanoparticles (AuNPs) were utilized as plasmonic imaging probes and dark field microscopy was applied to examine the movement as well as exocytosis of gold nanoparticles during cell migration. The results showed that the internalized AuNPs could localize into the retraction fibers. The mobility of nanoparticles reduced with the increased distance from the cell body. When the retraction fibers disconnected from the cell body, AuNPs were left outside the cell. This study demonstrated the migracytosis of nanomaterials, which could benefit the future design of safer and more efficient nanomaterials for diagnostics.
- Gold nanoparticles,
- Dark field imaging,
- Cell migration,
- Exocytosis
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