Citation: HU Xian-Li, ZHU Lin-Ling, LI Ling-Ling, XU Zhi-Ai, ZHANG Wen. Cationic Fluorescent Gold Nanoclusters for Nonviral siRNA Delivery[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(6): 712-720. doi: 10.19756/j.issn.0253-3820.201088 shu

Cationic Fluorescent Gold Nanoclusters for Nonviral siRNA Delivery

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

Received Date: 19 February 2020
Revised Date: 8 April 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21775046, 21675055).

RNA interference (RNAi) strategy based on small interfering RNA (siRNA) has become a standard method in biomedical research. In recent years, RNAi has also made remarkable advances in clinical translation. However, the conventional cationic vectors for siRNA delivery have severe disadvantages such as low delivery efficiency, high cytotoxicity and lack of imaging function, which severely restrict the clinical translation of siRNA drugs. To address the shortcomings of conventional siRNA vectors, we herein reported a cationic gold nanocluster (CGN)-based nanovectors for siRNA delivery and fluorescence imaging. Firstly, we used BSA template to synthesize GN by biomineralization strategy. Then, the BSA-templated GN was modified with ethylene diamine (EDA) or ethylene dimethyl amine (DMA) to form CGN. We demonstrated that the CGN can efficiently deliver siRNA and significantly silence green fluorescence protein (GFP) expression in A549-GFP lung cancer cells. Finally, we investigated the ability of CGN for fluorescence tracing in vitro by using confocal laser scanning microscopic examination. This study demonstrated the potential of the CGN for siRNA delivery and fluorescence tracing in vitro.
- Small interfering RNA delivery,
- Gold nanocluster,
- Cationic,
- Fluorescence tracing,
- Nanovector
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