Citation: YUAN Min, QIAN Shi-Quan, CAO Hui, XU Fei, YE Tai, YU Jin-Song, GUO Wen, WU Jia-Ying, A Ti-kan·Wu-siman. An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd²⁺[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(12): 1701-1708. doi: 10.19756/j.issn.0253-3820.201072 shu

An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd²⁺

YUAN Min ¹ ,
QIAN Shi-Quan ¹ ,
CAO Hui ¹ ,
XU Fei ^1,2,, ,
YE Tai ^1,2 ,
YU Jin-Song ^1,2 ,
GUO Wen ¹ ,
WU Jia-Ying ¹ ,
A Ti-kan·Wu-siman ¹

1.
School of Medical Instruments and Food Engineering, Shanghai Engineering Research Center for Food Rapid Detection, University of Shanghai for Science and Technology, Shanghai 200093, China;
2.
Institute of Food Quality and Safety, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: XU Fei, xufei8135@126.com
Received Date: 13 February 2020
Revised Date: 14 August 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 31671934, 61501295), the Ministry of Science and Technology of China (No. 2017YFC1600603) and the Shanghai Committee of Science and Technology (Nos.18391901200, 17391901500).

An aptamer-based electrochemical biosensor was developed for detection of cadmium ions (Cd²⁺). Firstly, the mercapto-modified complementary sequence (CDNA) of aptamer was immobilized on the electrode surface via AuS bond. Then, it hybridized with aptamer to form a double-stranded DNA. Due to the specific binding of aptamer and Cd²⁺, the aptamer labeled with ferrocene was competed off the gold electrode, so that the electrochemical signal of ferrocene decreased significantly. By using square wave voltammetry (SWV) detection and under the optimal conditions, a linear relationship was found between the current signal and logarithm of Cd²⁺ concentration with an equation of ΔI=0.2827+0.2372lgC (R²=0.9972), where the linearity range was from 1.0 nmol/L to 10.0 μmol/L, with a detection limit of 65.1 pmol/L, and the recoveries in real river samples were 97.1%-97.9%, which was comparable to those measured by graphite furnace atomic absorption spectrometry. The proposed method displayed many advantages such as high sensitivity, fast detection and excellent specificity, and had a good application prospect in monitoring cadmium pollution.
- Cadmium ion (II),
- Electrochemistry,
- Biosensor,
- Aptamer,
- Ferrocene
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An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd²⁺