Citation: ZHAO Ying, WANG Nan, GAO Hua-Long, GUO Zi-Xuan, LU An-Xiang, GUO Xiao-Jun, LU Jing-Hua, LUAN Yun-Xia. Determination of Aflatoxin B₁ in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 662-669. doi: 10.19756/j.issn.0253-3820.201030 shu

Determination of Aflatoxin B₁ in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

ZHAO Ying ^1,2, ,
WANG Nan ^1,2 ,
GAO Hua-Long ^2,3 ,
GUO Zi-Xuan ² ,
LU An-Xiang ² ,
GUO Xiao-Jun ² ,
LU Jing-Hua ¹ ,
LUAN Yun-Xia ²

1.
College of Pharmacy, Jinzhou Medical University, Jinzhou 121001, China;
2.
Beijing Research Center for Agricultural Standards and Testing, Agricultural Product Quality and Safety Risk Assessment Laboratory of the Department of Agriculture (Beijing), Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China;
3.
Capital Normal University, Beijing 100097, China

Received Date: 11 January 2020
Revised Date: 6 March 2020

Fund Project: This work was supported by the Science and Technology Innovation Special Construction Funded Program of Beijing Academy of Agriculture and Forestry Sciences (No.KJCX20170401) and the Beijing Agricultural Forestry Academy Youth Foundation (No. QNJJ201903).

An aptamer affinity column (AAC) was applied to specific identification and enrichment of aflatoxin B₁ (AFB₁). Aptamer was used as a novel recognition element for the high affinity and strong specificity of target. By optimizing the loading, washing and eluting conditions of AFB₁ in the sample, AFB₁ aptamer affinity column exhibited a good sample capacity (334.6±18.2) ng. The content of AFB₁ lotus seeds was detected by photo chemical reactor for enhanced high-performance liquid chromatography combined with photo chemical reactor for enhanced detection and fluorescence detection (HPLC-PCD-FLD) and AAC clean-up, with LOD of 0.05 ng/mL and excellent recoveries of 91.8%-108.6%. Meanwhile, the AAC could be reused at least twenty times. This study provides a new method for the enrichment and purification of trace toxins in complex matrix such as food, agricultural products and traditional Chinese medicines, and has a good application prospect.
- Aptamer affinity column,
- Aflatoxin B₁,
- Solid-phase extraction,
- N-Hydroxy succinimide-activated sepharose,
- Lotus seed
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沈阳化工大学材料科学与工程学院沈阳 110142

Determination of Aflatoxin B1 in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

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通讯作者: 陈斌, bchen63@163.com

Determination of Aflatoxin B₁ in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment