Citation: ZHAO Li-Ping, YANG Ge, ZHANG Xiao-Min, QU Feng. Development of Apatmer Screening Against Proteins and Its Applications[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 560-572. doi: 10.19756/j.issn.0253-3820.201024 shu

Development of Apatmer Screening Against Proteins and Its Applications

School of Life Science, Beijing Institute of Technology, Key Laboratory of Molecular Medicine and Biological Diagnosis, Ministry of Industry and Information Technology, Beijing 100081, China

Received Date: 9 January 2020
Revised Date: 25 February 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21675012, 21874010, 21827810).

Aptamers, short single stranded DNA or RNA, are selected through systematic evolution of ligands by exponential enrichment (SELEX), which can bind with target molecules with high affinity and high specificity. Proteins are vital functional macromolecules. Many SELEX techniques have been developed for screening of aptamers against proteins. In recent years, in order to obtain aptamers with high affinity and specificity, many SELEX methods have been developed to enhance selection efficiency, reduce cost and improve aptamer properties. Affinity characterization is also the key procedure in aptamer selection, which demonstrates if the obtained aptamer can satisfy the application requirement. In this review, the progress in aptamer screening for protein targets were summarized, and the optimization methods of nucleic acid library, improvement and development of screening methods and application of aptamers against proteins were mainly emphasized.
- Aptamer,
- Protein,
- Selection,
- Systematic evolution of ligands by exponential enrichment,
- Review
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