Citation: LU Xiang-Ting, ZHANG Shi-Ding, GONG Can, XU Xu. Separation and Determination of Triacylglycerols in Edible Oils by High Performance Liquid Chromatography with Porous Graphite Carbon Column and n-Octane-isopropanol Mobile Phase[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(8): 1084-1095. doi: 10.19756/j.issn.0253-3820.201009 shu

Separation and Determination of Triacylglycerols in Edible Oils by High Performance Liquid Chromatography with Porous Graphite Carbon Column and n-Octane-isopropanol Mobile Phase

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Received Date: 6 January 2020
Revised Date: 26 February 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31671928) and the Natural Science Foundation of Shanghai, China (No. 15ZR1440800).

High performance liquid chromatography with porous graphite carbon (PGC) columns and a n-octane-isopropanol mobile phase was used to separate triacylglycerols (TAGs) in edible oils. The samples were separated by a Hypercarb column (2.1 mm×100 mm, 5 μm) with n-octane-isopropanol (70:30, V/V) as mobile phase at a flow rate of 0.25 mL/min and column temperature of 60℃, with detection wavelength of 215 nm. The experimental conditions were optimized. Then, TAGs in 7 vegetable oils and 5 animal fats were separated under the selected experimental conditions. A total of 14 TAGs in corn oil, 9 TAGs in olive oil, and 14 TAGs in sunflower oil, 14 TAGs in soybean oil, 15 TAGs in sesame oil, 18 TAGs in peanut oil, 16 TAGs in rapeseed oil, 19 TAGs in chicken fat, 19 TAGs in lard, 12 TAGs in sheep fat, 13 TAGs in beef tallow and 16 TAGs in goose fat were identified by combining with mass spectrometry. By means of analysis of chicken fat and beef tallow, it was found that the SLP and SOP could be all separated with their respective isomers, which indicated that the system could separate the TAG isomers. The method was validated and applied to determine LLL in corn oil, rapeseed oil, sunflower seed oil, soybean oil, peanut oil, sesame oil and olive oil. The practical method used less-polluting mobile phase to provide a valuable method for the analysis of triacylglycerols and its isomers in edible oil.
- Porous graphite carbon column,
- High performance liquid chromatography,
- Mass spectrometry,
- Edible oils,
- Triacylglycerols,
- n-Octane-isopropanol mobile phase
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