Citation: SUN Miao, YANG Ge, ZHAO Yi, QU Feng. Screening of Aptamer for Breast Cancer Biomarker Calreticulin and Its Application to Detection of Serum and Recognition of Breast Cancer Cell[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 642-649. doi: 10.19756/j.issn.0253-3820.201004 shu

Screening of Aptamer for Breast Cancer Biomarker Calreticulin and Its Application to Detection of Serum and Recognition of Breast Cancer Cell

School of Life Science, Beijing Institute of Technology, Key Laboratory of Molecular Medicine and Biological Diagnosis, Ministry of Industry and Information Technology, Beijing 100081, China

Received Date: 3 January 2020
Revised Date: 5 March 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21675012, 21874010, 21827810).

Calreticulin (CRT) is closely related to cancer, and is considered to be a novel biomarker for invasion and metastasis of breast cancer, as well as an indicator of staging and prognosis. Here, we screened and identified aptamers of CRT for the first time with capillary electrophoresis-based systematic evolution of ligands by exponential enrichment (CE-SELEX). The high affinity and specificity Apt 23 was screened and verified by CE isothermal titration calorimetry and gold nanoparticles-based colorimetric assay. By using FAM-labeled Apt 23 (FAM-Apt 23) as an affinity probe, CRT was dected in the linear concentration range of 1-1000 nmol/L in serum by laser-induced fluorescence detector with a detection limit of 0.5 nmol/L. FAM-Apt 23 could also be used as an immunofluorescence imaging probe, which could be bound to the surface of breast cancer cells 4T1 with overexpressed CRT. Preliminary studies have shown that Apt 23 could be used as aptamer probes for recognition and detection of CRT.
- Calreticulin,
- Aptamer,
- Capillary electrophoresis based systematic evolution of ligands by exponential enrichment
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