Citation: JIANG Hao, LYU Xue-Fei, ZHAO Ke-Xin. Progress of Aptamer Screening Techniques Based on Microfluidic Chips[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 590-600,684. doi: 10.19756/j.issn.0253-3820.191775 shu

Progress of Aptamer Screening Techniques Based on Microfluidic Chips

School of Life Science, Beijing Institute of Technology, Beijing 100081, China

Received Date: 31 December 2019
Revised Date: 23 March 2020

Aptamer is a type of single-stranded DNA or RNA that can specifically bind with target molecules, and has been widely used in the fields of biological separation analysis, clinical diagnosis, and disease targeted therapy, and so on. The development of aptamers is closely related to the progress of screening techniques. Based on the Systematic evolution of ligands by exponential enrichment (SELEX), a variety of in vitro aptamer screening techniques such as magnetic beads SELEX and capillary electrophoresis SELEX have been developed to obtain specific and high-affinity aptamers for target molecules. However, these methods have many disadvantages such as multiple screening rounds, long screening cycles, and large sample consumption, or have difficult in screening small molecules. The microfluidic chip has the characteristics of small size, high throughput, and easy integration, which can solve the above-mentioned problems of SELEX to a certain extent, and realize the rapid and high-throughput screening of aptamers. Based on the summary of SELEX and their key points, this paper focuses on the research progress of SELEX techniques based on microfluidic and microarray chips, and finally, the aspects of SELEX needing to be strengthened are summarized and prospected.
- Aptamer,
- Screening,
- Systematic evolution of ligands by exponential enrichment,
- Microfluidic chip,
- Microarray chip,
- Review
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