Citation: YANG Yan, YIN Ying-Ai, DONG Yi-Yang. Detection of Aflatoxin B₁ in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 676-684. doi: 10.19756/j.issn.0253-3820.191728 shu

Detection of Aflatoxin B₁ in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

Received Date: 10 December 2019
Revised Date: 14 April 2020

Fund Project: This work was supported by the National Key Research and Development Program of China (No.2016YFF0203703).

Aflatoxin B₁ (AFB₁) is a toxic metabolite produced by fungi or molds, which can cause serious harm to human health. Based on the classic gold nanoparticles (AuNPs)-based colorimetric method, an AuNPs-aptasensor applicable for two different lengths of AFB₁ aptamers determining AFB₁ in corn oil was established in this study. The sensitivity of the AuNPs-aptasensor was further improved by optimizing the concentration of aptamer and NaCl and the reaction temperature. Under the optimal conditions, the limits of detection of AuNPs-aptasensor based on 50 (B50) and 80 (A80) base aptamers were 13.55 ng/mL and 10.56 ng/mL, respectively, with the linear range of 20-1000 ng/mL. Moreover, the AuNPs-aptasensor had good selectivity and the recoveries were 102.4%-104.9% and 98.1%-109.1%, respectively. The results indicated that AuNPs-aptasensor could be utilized as an efficient tool for AFB₁ identification in corn oil sample. The successful development of AuNPs-aptasensor provided a powerful support for rapid on-site detection and efficient screening of AFB₁ in food and showed the potential for construction of biosensors based on other targets and aptamers.
- Aflatoxin B₁,
- Aptamer,
- Colorimetric gold nanoparticles-aptasensor,
- Corn oil
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Aflatoxin B1 in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor

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通讯作者: 陈斌, bchen63@163.com

Detection of Aflatoxin B₁ in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor