Citation: YU Qing, LIU Ming-Zhu, XIAO He-He, YI Yi, CHENG Hao, Putra Dedi-Fazriansyah, LI Si-Qiao, LI Peng-Fei. Selection and Characterization of Aptamers for Specific Detection of Iridovirus Disease in Cultured Hybrid Grouper (Epinephelus Fuscoguttatus ♀×E. Lanceolatus ♂)[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 650-661. doi: 10.19756/j.issn.0253-3820.191647 shu

Selection and Characterization of Aptamers for Specific Detection of Iridovirus Disease in Cultured Hybrid Grouper (Epinephelus Fuscoguttatus ♀×E. Lanceolatus ♂)

YU Qing ^1,2, ,
LIU Ming-Zhu ¹ ,
XIAO He-He ^3,4 ,
YI Yi ² ,
CHENG Hao ² ,
Putra Dedi-Fazriansyah ⁵ ,
LI Si-Qiao ¹ ,
LI Peng-Fei ^1,2

1.
Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning 530007, China;
2.
Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China;
3.
Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences, Nanning 530007, China;
4.
College of Life Science, Henan Normal University, Xinxiang 453007, China;
5.
Department of Aquaculture, Syiah Kuala University, Banda Aceh 999006, Indonesia

Received Date: 3 November 2019
Revised Date: 9 February 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 41706145, 41966004), the Key Research and Development Programs of Guangxi Province, China (No. 2018AB52003), the Natural Science Foundation of Guangxi Province, China (Nos. 2019GXNSFAA245054, AD19245022), and the Basic Research Fund of Guangxi Academy of Sciences (Nos. 2019YJJ1005, 2018YJJ903, 2018YJJ902).

Grouper iridovirus is a nucleocytoplasmic DNA virus, which not only causes great economic losses in mariculture but also seriously threatens the global biodiversity. To further elucidate the molecular mechanism of grouper iridovirus pathogenesis, and provide the new theoretical basis for the prevention, diagnosis and treatment of grouper iridovirus disease, in this study, we generated four single-stranded DNA aptamers against SGIV-Gx infected cells using systematic evolution of ligands by exponential enrichment (SELEX). Then, the properties of aptamers were characterized. Firstly, each aptamer exhibited high specificity and affinity to grouper iridovirus Guangxi strain (SGIV-Gx)-infected cells. Secondly, aptamers exerted no cytotoxic effects, and aptamer LYGV3 appeared to inhibit SGIV-Gx infection in vitro. The target molecules of LYGV1, LYGV2, LYGV3, LYGV4 could be directly or indirectly related to surface proteins anchored on the membranes of SGIV-Gx infected cells. The targets of aptamers (LYGV1, LYGV2, LYGV3, LYGV4) appeared on the cells surface at 2, 4, 8, and 6 hour post infection.
- Grouper iridovirus,
- Systematic evolution of ligands by exponential enrichment,
- Aptamer,
- Specificity,
- Antiviral activity,
- Target protein
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
1. [1]
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