Citation: WEI Jia-Hui, CHEN Jia, WU Bi-Dong, CHEN Zuo-Hong, WU Jian-Feng, XIE Jian-Wei. Determination of Amanita Peptides in Human Plasma and Urine by High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(3): 405-412. doi: 10.19756/j.issn.0253-3820.191604 shu

Determination of Amanita Peptides in Human Plasma and Urine by High-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

1.
State Key Laboratory of Toxicology and Medical Countermeasures and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China;
2.
PLA 307 Hospital, Beijing 100071, China;
3.
College of Life Sciences, Hunan Normal University, Changsha 410081, China

Received Date: 14 October 2019
Revised Date: 31 December 2019

Five amanita peptide toxins, such as α-amanita, β-amanita, phalloidin, viroidin and ala-viroidin in human plasma and urine were determined by high-performance liquid chromatography coupled with triple quadrupole mass spectrometry under positive ion multiple reaction monitoring mode (MRM). The samples were enriched and purified by HLB solid phase extraction cartridge, and separated by CAPCELL CORE C₁₈ column using 10 mmol/L aqueous ammonium acetate-methanol as mobile phase under a gradient elution. The results indicated that the five amanita peptide toxins in plasma or urine had good linear relationship in a wide range of concentration levels. The detection limits of the toxins were 0.2 and 0.5 μg/L (S/N=3), the average recoveries were 98.0%-114.6% and 90.1%-99.4% for spiked plasma and urine samples, respectively. Accuracy, precision and matrix effects could meet the methodological requirement for biological samples analysis. The established method had many advantages such as simple operation and high sensitivity, which provided an accurate and reliable technical support for the diagnosis and medical treatment of clinical amanita poisoned patients.
- High performance liquid chromatography,
- Mass spectrometry,
- Amanita peptides toxins,
- Plasma,
- Urine
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