Citation: CHEN Piao-Piao, XING Yi-Chen, LIU Yang, ZHENG Shan, HUANG Chao-Biao. DNA Quantum Dots@Polydopamine as a Fluorescent Sensor for Cysteine Detection Based on Fluorescence Resonance Energy Transfer Effect[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(1): 83-89. doi: 10.19756/j.issn.0253-3820.191576 shu

DNA Quantum Dots@Polydopamine as a Fluorescent Sensor for Cysteine Detection Based on Fluorescence Resonance Energy Transfer Effect

1.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2.
Xingzhi College, Zhejiang Normal University, Lanxi 321100, China

Received Date: 28 September 2019
Revised Date: 10 November 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21575129).

A method for detecting cysteine based on fluorescenceresonance energy transfer (FRET) between DNA quantum dots (QDs) and polydopamine (PDA) was developed. Since the fluorescence emitted by DNA QDs was absorbed by the PDA molecule, FRET occurred, resulting in fluorescence quenching of DNA QDs, and leaving the DNA QDs in a fluorescent "off" state. In the presence of cysteine, the spontaneous oxidative polymerization from dopamine (DA) to PDA was blocked, the fluorescence of DNA QDs was restored, and the fluorescence was "on" state. Based on this, a cysteine fluorescence sensor was developed. The sensor had good selectivity to cysteine, and there was no interference between common amino acids and small biothiol molecules. The linear equation was y=0.0181x-0.0185 in linear range of 10.0-100.0 μmol/L. The limit of detection (LOD) was 1.7 μmol/L (S/N=3). The method was successfully applied to determination of cysteine in human urine samples, with recoveries of 98.6%-105.9%.
- DNA quantum dots,
- Fluorescence resonance energy transfer,
- Polydopamine,
- Cysteine
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1.
沈阳化工大学材料科学与工程学院沈阳 110142