Citation:
WEN Xue, ZHENG Hai-Jiao, ZHANG Yang, JIA Qiong. Progress in Monolithic Column-based Separation and Enrichment of Glycoproteins[J]. Chinese Journal of Analytical Chemistry,
;2020, 48(1): 13-21.
doi:
10.19756/j.issn.0253-3820.191566
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As a post-translational modification, protein glycosylation not only reflects on body health, but also serves as the target of drug treatment. Therefore, it is of great significance to study protein glycosylation for the diagnosis and treatment of diseases. However, many glycoproteins of biological importance exhibit relatively low abundance, high complexity, and wide dynamic range in biological samples. It is indispensable to explore highly efficient sample pretreatment methods. In recent years, monolithic columns have attracted extensive attention in the field of separation and enrichment of glycoproteins due to their advantages of rapid separation, high efficiency, and good biological compatibility. In this paper, recent progress of monolithic columns in the field of separation and enrichment of glycoproteins is surveyed, and the future development is briefed.
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Keywords:
- Monolithic column,
- Glycoprotein,
- Sample pretreatment,
- Separation,
- Enrichment,
- Review
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