Citation: BAI Chao-Peng, YANG Shao-Ming, TENG Yu, ZHANG Jian. Detection of Thrombin by Copper Ion Coordination-Molecular Imprinted Electrochemical Sensor Based on Graphene and Gold Nanoparticles[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(1): 137-144. doi: 10.19756/j.issn.0253-3820.191543 shu

Detection of Thrombin by Copper Ion Coordination-Molecular Imprinted Electrochemical Sensor Based on Graphene and Gold Nanoparticles

School of Materials Science and Engineering, East China Jiaotong University, Nanchang 330013, China

Received Date: 28 September 2019
Revised Date: 14 November 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21465012).

A metal ion ligands and molecular imprinted electrochemical sensors with double recognition patterns were prepared using molecular imprinting technique and with copper ion coordination unit as the double recognition mode. L-cystine (L-cys) was firstly assembled on the surface of graphene (rGO) and gold nanoparticles (AuNPs) modified glassy carbon electrode by AuS bond, and then the Cu-THR complex was assembled on the electrode surface by the coordination of L-cys and thrombin (Tn) by layer-by-layer self-assembly method. Thionine was used as monomer and electropolymerization method was used to form the imprinted membrane. The composite nanomaterials were characterized by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The performance of the sensor was studied by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Under the optimum conditions, the sensor showed an excellent linear relationship with thrombin concentration in the range of 2.0×10^-9-5.0×10^-7 g/L. The linear equation was -ΔI (μA)=17.73+1.84lgc (g/L). The kinetic adsorption model of THR molecularly imprinted electrochemical sensor was investigated, and the measured imprinting factor β of the imprinted sensor was 4.32, with the binding rate constant k of 5.68 s. The sensor had good stability and reproducibility, and could be used to detect thrombin in practice.
- Graphene,
- Gold nanoparticles,
- Thrombin,
- Double recognition mode,
- Molecular imprinted electrochemical sensor
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