Citation: LI Xin-Yu, ZHANG Qiang, WANG Ni, LIU Jia-Jun, WANG Jian. Cu²⁺-mediated Fluorescence Switching of Graphene Quantum Dots for Highly Selective Detection of Glutathione[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(3): 339-346. doi: 10.19756/j.issn.0253-3820.191493 shu

Cu²⁺-mediated Fluorescence Switching of Graphene Quantum Dots for Highly Selective Detection of Glutathione

LI Xin-Yu ^1,2, ,
ZHANG Qiang ¹ ,
WANG Ni ¹ ,
LIU Jia-Jun ¹ ,
WANG Jian ¹

1.
Key Laboratory of Luminescent and Real-Time Analytical System(Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China;
2.
Demonstration Center for Experimental Pharmacy Education(Southwest University), College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

Received Date: 11 August 2019
Revised Date: 18 November 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21405123), the Natural Science Foundation of Chongqing, China (No.cstc2019jcyj-msxmx0279), the Fundamental Research Funds for the Central Universities, China (No.XDJK2019AC002), and the College of Pharmaceutical Sciences Innovation and Entrepreneurship Project of Southwest University, China (No.YX2017-CXYB-10).

As a short peptide containing active thiol group, glutathione participates in many intracellular reactions, so it is of great significance to detect glutathione in cells. In this work, amino-rich graphene quantum dots were synthesized, which could coordinate with copper ions and yield aggregation-induced fluorescence quenching. However, copper ions and glutathione have stronger coordination ability, so that glutathione can promote the dissociation of copper ions from graphene quantum dots, and then lead to the fluorescence recovery of graphene quantum dots. In BR buffer media at pH 6.8, glutathione can gradually recover the fluorescence of graphene quantum dots (1 μg/mL) that is quenched by Cu²⁺ (250 μmol/L), which can finish within 20 min. Additionally, recovery degree of fluorescence signal is linear to the concentration of glutathione in the range of 20-500 μmol/L, and the detection limit is 3.4 μmol/L. This method is applicable to the detection of glutathione in cell lysate by using the switch action of copper ion to improve the selectivity.
- Graphene quantum dots,
- Glutathione,
- Copper ions,
- Fluorescence switching
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沈阳化工大学材料科学与工程学院沈阳 110142

Cu2+-mediated Fluorescence Switching of Graphene Quantum Dots for Highly Selective Detection of Glutathione

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通讯作者: 陈斌, bchen63@163.com

Cu²⁺-mediated Fluorescence Switching of Graphene Quantum Dots for Highly Selective Detection of Glutathione