Citation: CHEN Li-Pin, ZHANG Xiao-Mei, HU Ling-Ping, BI Shi-Jie, LIN Li-Ming, LI Zhao-Jie, ZHANG Hong-Wei, XUE Chang-Hu. Analysis of Changes of Endogenous Peptides of Live Pacific Oysters at Different Circulation Stages Based on Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(12): 1893-1900. doi: 10.19756/j.issn.0253-3820.191444 shu

Analysis of Changes of Endogenous Peptides of Live Pacific Oysters at Different Circulation Stages Based on Mass Spectrometry

1.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
2.
Qingdao Customs Technology Center, Qingdao 266002, China

Received Date: 25 July 2019
Revised Date: 21 October 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31871867), the National Quality Supervision, Inspection and Quarantine General Administration Science and Technology Plan Project (No. 2016IK204) and the Earmarked Fund for Modern Agro-industry Technology Research System (No. CARS-49).

To simulate the life-saving circulation of Pacific oysters, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF) was used to analyze the changes of endogenous peptides of live Pacific oysters at different circulation stages. Chemometric method was used to screen the differential peptide markers at each stage of circulation to obtain a set of new indicators for evaluating the quality changes in the circulation of live Pacific oysters. According to the peptide histology technique, the endogenous peptide was extracted from samples at 7 key time points in the whole process of circulation of live Pacific oysters (cleaning, temporary purification and nourishing), and then separated and identified by UHPLC-Q-TOF to obtain the peptide group profile at each circulation stage. Multivariate statistical analysis such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used for data mining and screening for differential markers. The online amino acid sequence was used to identify the amino acid sequences of differential peptides. The results showed that 3, 10, and 8 potential peptide markers were screened in the cleaning, temporary purification, and water-free preservation phases, respectively. The potential marker peptide DYDPVDK was selected for synthesis and the multi-reaction monitoring (MRM) analysis method based on liquid chromatography-triple quadrupole mass spectrometry was used to verify the correctness of the differential marker peptide sequence and its applicability for routine liquid chromatography analysis. In this work, peptide analysis was used for the first time to characterize the endogenous peptides of live Pacific oysters at different circulation stages. The differential marker peptides in different circulation stages were selected to provide the basis for detection of circulation process of live shellfish.
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沈阳化工大学材料科学与工程学院沈阳 110142