Citation: LU Ting-Ting, WANG Jin-Long, ZHAN Xiang-Qiang, WU Yuan-Gen. Carbon Dots-based Fluorescent Aptasensor for Detection of Dimethoate Pesticide[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(1): 74-82. doi: 10.19756/j.issn.0253-3820.191442 shu

Carbon Dots-based Fluorescent Aptasensor for Detection of Dimethoate Pesticide

1.
Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;
2.
Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry, Yibin 644000, China

Received Date: 25 July 2019
Revised Date: 15 November 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21565009, 31760486), the Natural Science Foundation of Guizhou Province (No.[2016]1403), the Science and Technology Support Program of Guizhou Province for Social Development (No.[2018]2795), and the Foundation of Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation (No. 2018JJ002), China National Light Industry.

A fluorescent aptasensor for detection of dimethoate pesticide based on aptamer regulated carbon dots was fabricated. In the presence of dimethoate, SS24-S-35 aptamer could alter the quenching efficiency of silver nanoparticles (AgNPs) on the fluorescence of carbon dots prepared from ethylenediamine and citric acid, and the degree of fluorescence quenching was proportional to the concentration of dimethoate molecules. Therefore, this strategy used the proposed aptasensor to quantitatively detect dimethoate pesticide by fluorescent assay. To improve the detection performance of the as-prepared aptasensor, the fluorescence quenching mechanism of AgNPs on carbon dots was fully discussed, and some parameters such as the types of carbon dots and the amount of AgNPs were investigated. Under optimal conditions, the aptasensor could detect dimethoate in a linear range from 6 μg/L to 200 μg/L (R²=0.984), with a limit of detection (LOD) as low as 2.24 μg/L (3σ/S). Moreover, the further studies confirmed that other pesticides had negligible effect on detection of dimethoate, indicating the excellent specificity of aptasensor for dimethoate detection. The aptasensor was succesfully used in detection of dimethoate pesticide in real agriculture products with recovery of 85.1%-105.0%, which indicated that the proposed aptasensor had potential application in the detection of pesticide residues.
- Pesticides detection,
- Aptamer,
- Silver nanoparticles,
- Carbon dots,
- Fluorescence resonance energy transfer
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沈阳化工大学材料科学与工程学院沈阳 110142