Citation: WANG Na, GU An, MIN Jun-Rong, LI Guang-Hua, LEI Yong. Identification of Protein Binding Media Used in Chinese Cultural Relics by Pyrolysis-Gas Chromatography/Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(1): 90-96. doi: 10.19756/j.issn.0253-3820.191355 shu

Identification of Protein Binding Media Used in Chinese Cultural Relics by Pyrolysis-Gas Chromatography/Mass Spectrometry

Department of Conservation Science, The Palace Museum, Beijing 100009, China

Received Date: 25 June 2019
Revised Date: 27 November 2019

Pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) has been more and more used in the qualitative investigation of organic materials in cultural relics, owing to its high sensitivity and wide range of application. However, there are few systematical studies on identifying species of different protein materials by Py-GC/MS. Therefore, porcine blood, animal glue and egg, which are all commonly used as binding media in ancient Chinese artworks and objects, such as polychrome, architecture and furniture, have been investigated in the present work by Py-GC/MS, and the characteristic components of each protein have been summarized. Based on the established Py-GC/MS procedure, it's found that some nitrogen-free pyrolysis products can be served as characteristic components for distinguishing porcine blood, animal glue and egg white. Nitrogen-contained pyrolysis products of porcine blood, animal glue and egg white can be mainly divided into pyrroles, nitriles, prolines and indoles, and the relative content of the four classes of products in different protein is distinctly different, so it can also be served as feature information for distinguishing porcine blood, animal glue and egg white. In addition, pyrolysis products of egg yolk are mainly fatty acids. The established Py-GC/MS procedure and summarized data were successfully applied to the identification of protein binding medium in two historical samples of Qin Dynasty, which were collected from the mortar of Polychrome Lacquered Table with "Kui Long" Pattern and the ground layer of gilding painting on the eaves of West Wei-Fang of Hall of Mental Cultivation respectively. Research results of the present work are easy to spread, and it's not only suitable for the rapid and accurate identification of different proteins in cultural relics, but also has reference significance in the research of other organic materials used in cultural relics.
- Pyrolysis-gas chromatography/mass spectrometry,
- Binding medium in cultural relics,
- Protein,
- Porcine blood,
- Animal glue,
- Egg
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