Citation: CHEN Mi, YUE Ren-Ye, LI Zhi, WANG Gang-Lin, MA Nan. Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(1): 40-48. doi: 10.19756/j.issn.0253-3820.191318 shu

Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China

Received Date: 4 June 2019
Revised Date: 14 November 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21475093, 21522506).

MicroRNA (miRNA) detection is of considerable significance in the diagnosis and treatment of cancer. The expression level of cancer-related miRNA differs significantly in various cancer cell types, and it remains challenging to detect miRNA at both high and low levels accurately using a conventional sensor with fixed limit of detection and narrow linear detecting range. Herein, three kinds of gold nanoparticle-quantum dots composite were constructed by reasonable DNA sequence design. By stacking these nanocomposites sequentially, three kinds of sensors of single, double and multiple amplifications based on entropy-driven catalytic circuits were successfully established. The product of the upper nanocomposite was served as the catalyst of the downstream nanocomposite to activate disassembly, inducing high-order amplification. With stacking a nucleic acid circuit of the sensor, the detection limit of the sensor was reduced by one order of magnitude. Finally, the nanosensor had a detection limit of fmol/L. By stacking entropy-driving nucleic acid circuits, a novel, dynamically tunable nanosensor was established for quantitatively detecting miRNA with different expression levels in cancer cells.
- miRNA,
- Gold nanoparticles,
- Quantum dots,
- Nucleic acid circuit,
- Fluorescence detection
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