Highly Selective Separation of Gastrodin by SiO₂/Rosin-Cardano Core-Shell Liquid Chromatography Stationary Phase

LI Guo-Xiang ^1, ,
DENG Jie ^1,2 ,
ZENG Lei ¹ ,
SHI Bo-An ¹ ,
LEI Fu-Hou ²

1.
School of Chemical and Environmental Engineering, Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China;
2.
School of Chemistry and Chemical Engineering, Guangxi key Laboratory of Chemistry and Engineering of Forest Products, Guangxi University for Nationalities, Nanning 530006, China

Received Date: 7 May 2019
Revised Date: 15 October 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31460172), the Hubei Key Laboratory of Biological Resources Protection and Utilization for the Open Fund (Hubei Minzu University, No. PKLHB1716), the Guangxi key Laboratory of Chemistry and Engineering of Forest Products for the Open Fund of Guangxi University for Nationalities (No. GXFC14-02) and the Special Funds for "Double First-Class"Construction in Hubei Province, China.

SiO₂/rosin-cardanol core-shell high performance liquid chromatography column was prepared with cardanol, rosin and spherical silica as main raw materials for purification of gastrodin. In the method, cardanol was esterified to prepare cardanol-ester as monomer, modified rosin and azodiisobutyronitrile (AIBN) were used as crosslinker and initiator, respectively. They were mixed and evenly coated on the surface of the silanized silica gels and polymerized in situ. The stationary phases were characterized by Fourier transform infrared sepctroscopy, scanning electron microscopy and transmission electron microscopy, and were packed in stainless steel hollow column by wet process. Phenyl-β-D-glucopyranoside and 4-methoxyphenyl-β-D-glucopyranoside were selected as competitors of gastrodin, acetonitrile-0.05% phosphoric acid aqueous solution (3:97, V/V) was used as mobile phase, the detection wavelength was set at 220 nm, the flow rate was 0.3 mL/min, and the separation performance about gastrodin of the core-shell stationary phase was explored at room temperature. The results showed that the particle size of the stationary phases was about 5 μm, the size distribution was uniform, and the thickness of shell (membrane) was about 75 nm. The resolution (R_s) of gastrodin/phenyl-β-D-glucopyranoside and gastrodin/4-methoxyphenyl-β-D-glucopyranoside was 7.43 and 12.35 respectively, which showed that the stationary phase had a higher selectivity to gastrodin. The method was simple and efficient, and provided a new way for separation of gastrodin.

Highly Selective Separation of Gastrodin by SiO₂/Rosin-Cardano Core-Shell Liquid Chromatography Stationary Phase

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通讯作者: 陈斌, bchen63@163.com

Highly Selective Separation of Gastrodin by SiO2/Rosin-Cardano Core-Shell Liquid Chromatography Stationary Phase

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通讯作者: 陈斌, bchen63@163.com

Highly Selective Separation of Gastrodin by SiO₂/Rosin-Cardano Core-Shell Liquid Chromatography Stationary Phase