Citation: NIU Wei-Fen, ZHANG Chao, JIA Juan, YUN Ke-Ming, SHUANG Shao-Min, DONG Chuan, WONG Man Shing. Synthesis and Properties of Fluorescent Probe for Detection of Biothiols in Live Cells[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(11): 1767-1775. doi: 10.19756/j.issn.0253-3820.191237 shu

Synthesis and Properties of Fluorescent Probe for Detection of Biothiols in Live Cells

1.
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China;
2.
Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3.
Department of Chemistry and Institute of Molecular Functional Materials, Hong Kong Baptist University, Hong Kong, China

Received Date: 27 April 2019
Revised Date: 14 August 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21705105).

A highly sensitive biothiols-selective fluorescent probe (MCY-NBD) was developed based on the merocyanine fluorophore of 4-(4-hydroxy styryl)-1-methyl pyridine iodide (MCY) and nitro benzo furazan (NBD) as the biothiols-specific recognition group. Upon reacting with biothiols, the probe showed a strong fluorescence enhancement more than 8-fold and very fast response. Moreover, the fluorescence intensity change of MCY-NBD at 526 nm was found to be linearly proportional to biothiols concentrations in the range of 0.1-4.0 μmol/L with a detection limit of 0.03 μmol/L, indicating that MCY-NBD was highly sensitive to biothiols. Confocal laser scanning microscopic imaging experiment proved that MCY-NBD had excellent biocompatibility and cell membrane permeability with low cytotoxicity, which was desirable for fast detection and visualization of both endogenous and exogenous biothiols in live cells.
- Biothiols,
- Fluorescent probe,
- Cells imaging
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沈阳化工大学材料科学与工程学院沈阳 110142