Citation: SHANG Ting, ZHAO Ling-Juan, LI Pei, ZENG Xiang-Ying, YU Zhi-Qiang. Determination of 10 Kinds of Monohydroxylated Polycyclic Aromatic Hydrocarbons in Human Urine by Supported Liquid Extraction Followed by Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(6): 876-882. doi: 10.19756/j.issn.0253-3820.191069 shu

Determination of 10 Kinds of Monohydroxylated Polycyclic Aromatic Hydrocarbons in Human Urine by Supported Liquid Extraction Followed by Liquid Chromatography-Tandem Mass Spectrometry

1.
State Key Laboratory of Organic Geochemistry, Guangdong Key Laboratory of Environment and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 30 January 2019

Fund Project: This work was supported by the "Strategic Priority Research Program" of the Chinese Academy of Sciences (No. XDB14010202), and the National Key Research and Development Program of China (No. 2017YFC0212003).

The monitoring of urinary monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) can be used for the risk assessment of human exposure to polycyclic aromatic hydrocarbons (PAHs). In this work, we developed and optimized a new pretreatment method based on solid-phase supported liquid-liquid extraction (SLE). After pretreatment, the analytes were then determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Urine samples were extracted with a mixed solution of dichloromethane and n-hexane (3:7, V/V) by a SLE clean-up using a diatomaceous earth mini-cartridge. The results indicated that the developed pretreated procedure had good recoveries and also significantly reduced the interference of biological matrix. At three concentration levels (10, 50 and 100 μg/L), the recoveries of ten OH-PAHs ranged from 88.5% to 120.9%, and the repeatability ranged from 1.6% to 8.1%. The limits of detection ranged from 0.06 μg/L to 0.3 μg/L and the limits of quantitation were between 0.2 μg/L and 1.0 μg/L. This method was finally successfully applied to the determination of OH-PAHs in urines collected from primary school students in Guangdong. The results showed that 2-OHN was the dominant compound, with the highest concentration of 4.83 μg/L.
- Monohydroxylated polycyclic aromatic hydrocarbons,
- Urine,
- Supported liquid extraction,
- Liquid chromatography-tandem mass spectrometry
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沈阳化工大学材料科学与工程学院沈阳 110142