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Citation: CHEN Meng-Di, YANG Yan-Ting, DENG Zhi-Yang, XU Hong-Yan, DENG Ji-Nan, YANG Zhong, HU Ning, YANG Jun. Microchannel with Stacked Microbeads for Separation of Plasma from Whole Blood[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(5): 661-668. doi: 10.19756/j.issn.0253-3820.181800 shu

Microchannel with Stacked Microbeads for Separation of Plasma from Whole Blood

1.
Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China;
2.
Bishan Middle School of Chongqing City, Chongqing 402760, China;
3.
College of Mechanical Engineering, Donghua University, Shanghai 200051, China;
4.
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China

Received Date: 24 December 2018
Revised Date: 31 January 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 81501617, 81871450, 21827812), the Program of International Science & Technology Cooperation (No. 2014DFG31380) and the Shanghai Pujiang Program (No. 18PJ1400200).

A microfluidic device integrated with a microchannel stacked by two kinds of microbeads with different sizes and a capillary microchannel array was designed and fabricated. When whole blood sample flowed through these channels, blood cells were filtered and adsorbed by the stacked microbeads, and plasma could be separated rapidly. The microbeads were introduced into the channel by negative pressure and thus stacked compactly. Protein blocking solution was used to improve the hydrophilicity of the bead surface. Then the microchip was dried and cooled before 20 μL blood was dropped in the inlet. Driven by capillary force, the blood proceeded in the channel. The influences of microbeads with different sizes and a locally widened microchannel were investigated. Experimental results showed that, when microbeads with 10 μm in diameter were filled in the locally widened microchannel, the separation rate of plasma was the fastest. The collection rate of plasma could reach up to 0.16 μL/min in this case, which could meet the requirement of most clinical applications. On the chip device, collected plasma was used to carry out the agglutination test, and the blood group could be determined quickly, which verified the usefulness of this method.
- Plasma,
- Microfluidic chip,
- Whole blood,
- Capillarity,
- Filter
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1.
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