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Citation: WANG Xia, XU Can, WU Xiu-Yuan, SUN Wen-Ting, WANG Ying-Feng, LI Zhong-Feng. Nuclear Magnetic Resonance-based Metabonomic for Study of Feces from Rats Treated with Root of Euphorbia Fischeriana Steud Extract[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(5): 669-677. doi: 10.19756/j.issn.0253-3820.181775 shu

Nuclear Magnetic Resonance-based Metabonomic for Study of Feces from Rats Treated with Root of Euphorbia Fischeriana Steud Extract

Department of Chemistry, Capital Normal University, Beijing 100048, China

Received Date: 12 December 2018
Revised Date: 19 March 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81573832).

Nuclear magnetic resonance (NMR)-based metabonomics was applied to analyze the alterations of fecal metabolites induced by Euphorbia fischeriana Steud (E. fischeriana). A total of 36 SPF male Sprague-Dawley rats were randomly divided into three groups. The administered groups were intraperitoneally injected with different dosages of the extract, while the control group was given the same volume of normal saline containing 3% Tween 80. Rats were administered once a day for 15 consecutive days and withdrawal period lasted for 15 days, and the fecal samples of rats in each group were collected. NMR technology was applied to analyze the fecal samples of rats. The obtained ¹H-NMR data were subjected to multivariate statistical analysis to select the altered endogenous metabolites in rat feces, and student test followed by multivariate statistical analysis to obtain the statistically significant biomarkers. Consequently, the metabolic signature of feces exhibited increased levels of glucose and butyrate, together with decreased levels of leucine, alanine, lysine, glutamate, methionine, aspartate, trimethylamine, dimethylamine, phenylalanine, and uracil in E. fischeriana extract administered groups. It could be seen that the significant altered metabolites associated with the metabolism of intestinal flora were futher induced disturbance in amino acid metabolism, short-chain fatty acids and glucose metabolism. The results provided experimental evidence to elucidate toxicity of E. fischeriana.
- Euphorbia fischeriana Steud,
- Metabonomics,
- Feces,
- ¹H-Nuclear magnetic resonance
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