Citation: GAO Fan, WANG Xiao-Fei, ZHANG Bo. Research and Application Progress of Micellar Electrokinetic Chromatography in Separation of Proteins[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(6): 805-813. doi: 10.19756/j.issn.0253-3820.181742 shu

Research and Application Progress of Micellar Electrokinetic Chromatography in Separation of Proteins

College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China

Received Date: 27 November 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21475110, 21535007, J1310024), the China PostDoc Foundation (No. 2018M642572), the Xiamen Municipal Science and Technology Plan Project (No. 3502Z20173019) and the Fundamental Research Funds for Central Universities of China (No. 20720160051).

Micellar electrokinetic chromatography (MEKC) is a hybrid of chromatography and electrophoresis. The separation mechanism is based on the interaction of analytes and pseudo-stationary phase and also the differential mobility in the electric field. Such property of MEKC can be made good use for separations of proteins and peptides, which have high dimensionality. In the past years, either in the form of single dimensional or multi-dimensional separations, MEKC demonstrated its excellent usability in biomolecule separations. Recently, as the development in CE-MS was revisited, there were also new developments in MEKC-MS hyphenation techniques, which further pointed to the usefulness of MEKC in analysis of proteins and peptides. In this article, the recent advances of MEKC applied in separation and analysis of proteins were reviewed and the prospect of this technique was anticipated.
- Micellar electrokinetic chromatography,
- Surfactant,
- Capillary electrophoresis,
- Proteins,
- Peptides,
- Mass spectrometry,
- Review
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