Home

Citation: YUAN Shuai, HUO Bing-Yang, ZHANG Man, WANG Yu, BAI Jia-Lei, PENG Yuan, NING Bao-An, CHEN Ping, GAO Zhi-Xian. Dual Signal Amplification Strategy for Detection of Ricin Toxin through Bio-Barcode Assay Combined with Hybridization Chain Reaction[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(1): 147-154. doi: 10.19756/j.issn.0253-3820.181504 shu

Dual Signal Amplification Strategy for Detection of Ricin Toxin through Bio-Barcode Assay Combined with Hybridization Chain Reaction

YUAN Shuai ^1,2, ,
HUO Bing-Yang ² ,
ZHANG Man ² ,
WANG Yu ² ,
BAI Jia-Lei ² ,
PENG Yuan ² ,
NING Bao-An ² ,
CHEN Ping ¹ ,
GAO Zhi-Xian ²

1.
Food Science and Engineering College, Jilin Agricultural University, Changchun 130118, China;
2.
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Environmental and Operational Medicine, Academy of Military Medical Science, Academy of Military Science, Tianjin 300050, China

Received Date: 2 August 2018
Revised Date: 30 September 2018

Fund Project: This work was supported by the National Basic Research and Development Plan of China (No. 2017YFC1200903) and the Tianjin Science and Technology Support Key Project (No. 18YFZCNC01260).

A novel highly sensitive and specific method for detection of ricin toxin (RT) based on bio-barcode combined with hybridization chain reaction (HCR) amplification was developed. Functional gold nanoparticles (GNPs) were coated with anti-ricin polyclonal antibodies (pAb) and barcode DNA. The "mAb-RT-pAb/AuNPs/barcode DNA" sandwich structure was formed by co-identification with the anti-ricin monoclonal antibody (mAb), and HCR was further carried out by using barcode DNA as an initiation chain probe for triggering the chain-triggered biotin label, which resulted in a long double-stranded DNA with repeating units. Immediately, streptavidin labeled horseradish peroxidase (SA-HRP) was combined with biotin on long strand DNA. Finally, the chemiluminescence values were detected through adding substrates of luminol and hydrogen peroxide. The results showed that the linear range of RT was 0.61 ng/mL-5000 ng/mL, and the limit of detection (LOD) was 0.52 ng/mL(S/N=3). This method showed a wide detection range and a low detection limit. The recoveries of spiked samples in milk and water samples ranged from 83.4% to 112.4%, and the relative standard deviations (RSDs) ranged from 2.2% to 5.4%. This method exhibited great application prospects in food drinking water safety control and prevention of bioterrorism attacks.
- Ricin toxin,
- Bio-barcode,
- Hybridization chain reaction,
- Streptavidin-biotin,
- Chemiluminescence
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
1. [1]
  Borong Yu , Huijiao Zhang , Xinyu Zhang , Xiaoying Li , Shuming Chen , Zhangang Han . The Blue Elf in the Dark: Gradient Science Popularization Experiments on Chemiluminescence. University Chemistry, 2024, 39(9): 295-303. doi: 10.12461/PKU.DXHX202403107
2. [2]
  Minna Ma , Yujin Ouyang , Yuan Wu , Mingwei Yuan , Lijuan Yang . Green Synthesis of Medical Chemiluminescence Reagents by Photocatalytic Oxidation. University Chemistry, 2024, 39(5): 134-143. doi: 10.3866/PKU.DXHX202310093
3. [3]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085
4. [4]
  Tianyu Feng , Guifang Jia , Peng Zou , Jun Huang , Zhanxia Lü , Zhen Gao , Chu Wang . Construction of the Chemistry Biology Experiment Course in the Chemistry “101 Program”. University Chemistry, 2024, 39(10): 69-77. doi: 10.12461/PKU.DXHX202409002
5. [5]
  Jianfeng Yan , Yating Xiao , Xin Zuo , Caixia Lin , Yaofeng Yuan . Comprehensive Chemistry Experimental Design of Ferrocenylphenyl Derivatives. University Chemistry, 2024, 39(4): 329-337. doi: 10.3866/PKU.DXHX202310005
6. [6]
  Zhibei Qu , Changxin Wang , Lei Li , Jiaze Li , Jun Zhang . Organoid-on-a-Chip for Drug Screening and the Inherent Biochemistry Principles. University Chemistry, 2024, 39(7): 278-286. doi: 10.3866/PKU.DXHX202311039
7. [7]
  Zhongyan Cao , Shengnan Jin , Yuxia Wang , Yiyi Chen , Xianqiang Kong , Yuanqing Xu . Advances in Highly Selective Reactions Involving Phenol Derivatives as Aryl Radical Precursors. University Chemistry, 2025, 40(4): 245-252. doi: 10.12461/PKU.DXHX202405186
8. [8]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
9. [9]
  Ying Zhang , Fang Ge , Zhimin Luo . AI-Driven Biochemical Teaching Research: Predicting the Functional Effects of Gene Mutations. University Chemistry, 2025, 40(3): 277-284. doi: 10.12461/PKU.DXHX202412104
10. [10]
  Qin Li , Kexin Yang , Qinglin Yang , Xiangjin Zhu , Xiaole Han , Tao Huang . Illuminating Chlorophyll: Innovative Chemistry Popularization Experiment. University Chemistry, 2024, 39(9): 359-368. doi: 10.3866/PKU.DXHX202309059
11. [11]
  Yixuan Zhu , Qingtong Wang , Jin Li , Lin Chen , Junlong Zhao . Blog of Oxytocin. University Chemistry, 2024, 39(9): 134-140. doi: 10.12461/PKU.DXHX202310090
12. [12]
  Meiqing Yang , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-. doi: 10.3866/PKU.WHXB202310046
13. [13]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
14. [14]
  Zongyuan Chen , ChunSheng Shi , Yiwen Li , Ganlin Zu , Qiang Jin , Haishan Wang , Fujun Wang , Dekun Yan , Zhijun Guo , Wangsuo Wu . Measurement of Uranium Isotopes in Environmental Water Samples by Alpha-Spectroscopy: Design of an Undergraduate Radiochemistry Experiment. University Chemistry, 2025, 40(4): 353-358. doi: 10.12461/PKU.DXHX202406103
15. [15]
  Yuyang Xu , Ruying Yang , Yanzhe Zhang , Yandong Liu , Keyi Li , Zehui Wei . Research Progress of Aflatoxins Removal by Modern Optical Methods. University Chemistry, 2024, 39(11): 174-181. doi: 10.12461/PKU.DXHX202402064
16. [16]
  . . Chinese Journal of Inorganic Chemistry, 2024, 40(12): 0-0.
17. [17]
  Luhong Chen , Yan Zhang . Chem&Bio Interdisciplinary Graduates Training in Nanjing University Promoted by Chemistry and Biomedicine Innovation Center. University Chemistry, 2024, 39(6): 12-16. doi: 10.3866/PKU.DXHX202311089
18. [18]
  Yukun Xing , Xiaoyu Xie , Fangfang Chen . A Sunlit Gift: Vitamin D. University Chemistry, 2024, 39(9): 28-34. doi: 10.12461/PKU.DXHX202402006
19. [19]
  Jijun Sun , Qianlang Wang , Qian Chen , Quanqin Zhao , Shumei Zhai . The Antibiotic Legion’s Manifesto to Human Allies. University Chemistry, 2025, 40(4): 307-321. doi: 10.12461/PKU.DXHX202405206
20. [20]
  Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two Ln^Ⅲ₃ complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188

Get Citation

PDF

XML

Metrics

PDF Downloads(14)
Abstract views(461)
HTML views(94)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return