Citation: HUANG Li-Ying, FAN Dong-Jie, ZHANG Yue-Xing, MENG Chun-Ying, GU Jie. Application of Multi-element and Stable Isotope Ratios in Geographical Traceability of Trichiurus haumela Sold Online[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(3): 439-446. doi: 10.19756/j.issn.0253-3820.181422 shu

Application of Multi-element and Stable Isotope Ratios in Geographical Traceability of Trichiurus haumela Sold Online

1.
Key Laboratory of Mariculture and Enhancement of Zhejiang Province, Marine Fisheries Research Institute of Zhejiang Province, Marine and Fishery Institute of Zhejiang Ocean University, Zhoushan 316021, China;
2.
Food and Medicine College of Zhejiang Ocean University, Zhoushan 316022, China;
3.
National Engineering Research Center of Marine Aquaculture, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China

Received Date: 26 June 2018
Revised Date: 21 December 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31502182).

To explore the possibility of applying the characteristics of element composition and stable isotopes of Trichiurus haumela sold online for investigation of traceability of geographic origin, the stable isotope ratios (δ¹³C and δ¹⁵N) and multi-element (Na, Mg, K, Ca, Mn, Fe, Cu, Zn, As, Sr) contents of 24 batches of trichiurus haumela in different regions including Lvsi Fishing Ground, Zhoushan Fishing Ground, Qinghai Fishing Ground, and Minnan Fishing Ground were determined by liquid chromatography-isotope mass spectrometry (LC-IRMS) and inductively coupled plasma mass spectrometry (ICP-MS). Based on the differences in their stable isotope ratios and elemental composition, a discriminant model was established based on cluster analysis (CA) and principal component analysis (PCA) to trace the origin of trichiurus haumela sold online in the four regions. Analysis of variance (ANOVA) showed that the stable isotope ratios and elemental composition of trichiurus haumela sold online in each area had obvious regional characteristics, and the contents of the nine elements were significantly different. CA result indicated that the correct discrimination rate of the sample origin was 83.33%. PCA showed that the scattered points of the first three principal components (the cumulative variance contribution rate is 82.16%) were divided into four relatively concentrated areas without any overlap with each other, and could effectively distinguish the origin of trichiurus haumela sold online. In summary, the use of stable isotopes and multi-element detection combined with CA, PCA could effectively trace the origin and discriminate of trichiurus haumela sold online, had certain feasibility and application value.
- Trichiurus haumela sold online,
- Stable isotopes,
- Multi-elements,
- Cluster analysis,
- Principal components analysis,
- Geographical origin
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沈阳化工大学材料科学与工程学院沈阳 110142