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Citation: WANG Li-Ying, BIAN Liang-Qi, SU Xiao-Wei, SU Hai-Yan, LIU Xu, LIU Nan-Nan. Analysis of Effect of Taurine on Myofibroblasts of Neonatal Rats[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(2): 256-261. doi: 10.19756/j.issn.0253-3820.181403 shu

Analysis of Effect of Taurine on Myofibroblasts of Neonatal Rats

1.
Physiology Department of Medicine College of Beihua University, Jilin 132013, China;
2.
Preventive Health Department of Tumor Hospital of Jilin Province, Changchun 130021, China

Received Date: 19 June 2018
Revised Date: 12 December 2018

Fund Project: This work was supported by the Science and Technology Research Projects of the Department of Education of Jilin Province (No.JJKH20190625KJ), the Science and Technology Development Projects of Jilin City, China (No.201536058) and the Start-up Fundation for Doctors (No.199500033).

The effect of taurine (Tau) on inhibiting the proliferation of myofibroblasts (myoFbs) of neonatal rats were studied, and the inhibiting mechanism was explored with the combination of different analytical techniques. Angiotensin Ⅱ (AngⅡ) was used to induce the proliferation of myoFbs in cultured neonatal rats, and myofibrosis mode was established in the experiment. The proliferation of myoFbs was detected based on methyl thiazolyl tetrazolium (MTT) assay, and the results showed that different concentrations of Tau (0.03, 0.06, 0.12 mol/L) could significantly inhibit myoFbs proliferation in a dose-dependent way. Furthermore, the expression and contents of reactive oxygen species (ROS), cell cycle protein E (CyclinE), nuclear factor-kappa Bp65(NF-κBp65) and transforming growth factor-β₁(TGF-β₁) were determined based on the immunocytochemistry, laser confocal scanning microscopy and ELISA kit method. It was found that Tau could decrease the content of ROS and the expression of TGF-β₁, and inhibit the translocation and expression of NF-κBp65 and CyclinE in nucleus, which resulted in the inhibition of proliferation of myoFbs and the effect of anti-fibrosis.
- Taurine,
- Myofibroblastst,
- Methyl thiazolyl tetrazolium assay,
- Reactive oxygen species,
- Cell cycle protein E
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