Citation: YANG Yin-Ping, LYU Guang-Fu, ZHANG Qiao, YAO Meng-Jie, LIANG Xin-He, LI Jing, ZHANG Hui, SUN Jia-Ming. Metabolomics Study of Protection Mechanism of Ginsenoside Rb₁ on Injury of SH-SY5Y Cell Induced by Glutamic Acid[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(1): 49-58. doi: 10.19756/j.issn.0253-3820.181149 shu

Metabolomics Study of Protection Mechanism of Ginsenoside Rb₁ on Injury of SH-SY5Y Cell Induced by Glutamic Acid

Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China

Received Date: 10 March 2018
Revised Date: 20 September 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 31570347, 81704001).

Metabolomics based on ¹H nuclear magnetic resonance (NMR) was established to explore the protection mechanism of ginsenoside Rb₁ on injury of SH-SY5Y cell induced by glutamic acid (Glu). Cell viability was evaluated by methyl thiazolyl tetrazolium (MTT) method, apoptotic rates were analyzed with PI/Annexin V flow cytometry, and the dynamic changes of cell metabolism were studied by using ¹H NMR technology platform and high-throughput multivariate statistical analysis to reveal the changes of endogenous metabolites of SH-SY5Y cell. It was found that ginsenoside Rb₁ could protect the SH-SY5Y cell by reducing apoptosis induced by glutamate and 7 metabolites of glutamic acid, taurine, acetic acid, arginine, creatine alanine, and proline were identified through orthogonal partial least squares discriminant analysis (OPLS-DA). Metaboanalyst showed taurine and hypotaurine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, D-Glutamine and D-glutamate metabolism and pyruvate metabolism were mainly involved. The results showed that ginsenoside Rb₁ had a protective effect on SH-SY5Y cell by affecting various energy and amino acid metabolism.
- Ginsenoside Rb₁,
- Metabolomics,
- ¹H nuclear magnetic resonance,
- Apoptosis protection,
- Orthogonal partial least squares discriminant analysis
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通讯作者: 陈斌, bchen63@163.com

Metabolomics Study of Protection Mechanism of Ginsenoside Rb₁ on Injury of SH-SY5Y Cell Induced by Glutamic Acid