Citation: Bing WANG, Qiong WU, JIN-Feng XIA, Xin LI, Dan-Yu JIANG, Qiang LI. Anthrax Biomarker: a Fluorescent Probe for Dipicolinic Acid Using Eu(Ⅲ)-doped Nanosheets[J]. Chinese Journal of Structural Chemistry, ;2020, 39(10): 1788-1794. doi: 10.14102/j.cnki.0254–5861.2011–2707 shu

Anthrax Biomarker: a Fluorescent Probe for Dipicolinic Acid Using Eu(Ⅲ)-doped Nanosheets

Bing WANG ^a ,
Qiong WU ^b ,
JIN-Feng XIA ^c ,
Xin LI ^a ,
Dan-Yu JIANG ^c ,
Qiang LI ^a,,

a.
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China
b.
Department of Chemistry, Hebei Normal University for Nationalities, Chengde, Hebei 067000, China
c.
Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China

Corresponding author: Qiang LI, qli@chem.ecnu.edu.cn
Received Date: 20 December 2019
Accepted Date: 21 February 2020

Fund Project: the Department of Science and Technology of Hebei Province 17272610

Figures(7)

A novel two-dimensional nanosheet fluorescent probe embedded with rare earth Eu(Ⅲ) was developed to detect dipicolinic acid (DPA), a biomarker of Bacillus anthracis bacterial spores. DPA, a major component of Bacillus anthracis spores which were highly toxic to humans, was not found in other common bacteria. The ability to detect ultra-low concentrations of DPA would therefore be of great significance. Eu(Ⅲ)-doped ytterbium hydroxide nanosheets were obtained by mechanical exfoliation from layered rare-earth hydroxide (LRH) materials. The crystallinities, layered structure and morphology of the as-synthesized nanosheets were studied by power X-ray diffraction, transmission electron microscopy and atomic force microscopy. Eu(Ⅲ) emission increased linearly with DPA addition in the range of 0.1~30 μmol/L. Based on the antenna effect, the detection limit of DPA was 0.078 μmol/L and much lower than the infective dose of Bacillus anthracis in humans of 60 μmol/L. The nanosheet fluorescent probe exhibited good specificity toward DPA, and the interferences with selected aromatic ligands and amino acids were observed to be negligibly small in comparison with that of DPA. Our findings provide a basis for the application of Eu(Ⅲ)-doped nanosheets for accurate, sensitive, and selective monitoring of DPA as a biomarker of anthrax.
- layered compound,
- nanosheets,
- fluorescent probe,
- dipicolinic acid,
- bacterial spores
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