Citation: Kun YU, Ying LI, Hai-Jiao CHEN, Bo LIU, Qing-Qiang YAO. Biological Activities and Crystal Structure of the Natural Anti-cancer Drug: Cucurbitacin IIa[J]. Chinese Journal of Structural Chemistry, ;2020, 39(7): 1283-1287. doi: 10.14102/j.cnki.0254–5861.2011–2608 shu

Biological Activities and Crystal Structure of the Natural Anti-cancer Drug: Cucurbitacin IIa

Kun YU ^{a, b, ②} ,
Ying LI ^{b, ②} ,
Hai-Jiao CHEN ^{a, b} ,
Bo LIU ^b,, ,
Qing-Qiang YAO ^b,,

a.
School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250200, China
b.
Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China

Corresponding author: Bo LIU, mls_liub@ujn.edu.cn Qing-Qiang YAO, yao_imm@163.com
Received Date: 20 September 2019
Accepted Date: 14 January 2020

Fund Project: the National Natural Science Foundation of China 81903473

Figures(2)

We isolated cucurbitacin IIa from the rhizomes of Hemsleya pengxianensis, and tested the cytotoxicity of cucurbitacin IIa against various cancer cell lines by the sulforhodamine B assay (SRB). At the same time, we preliminarily found that cucurbitacin IIa has a certain inhibitory activity on kinase CDK1/cyclin B, and shows potent inhibitory activities against many cancer cell lines. The cucurbitacin IIa was structurally characterized by specific optical rotation measurement, high-resolution mass spectroscopy and NMR spectroscopic analysis. In addition, the molecular structure of cucurbitacin IIa was further determined by X-ray single-crystal crystallography.
- cucurbitacin IIa,
- crystal structure,
- sulforhodamine B assay,
- CDK1/cyclin B,
- anti-cancer activity
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