Citation: Houjin Li, Lin Wu, Xingwen Sun, Yuan Zheng, Zhanxiang Liu, Shuanglian Cai, Ying Xiong, Guangao Yu, Qingwen Liu, Jie Han, Xin Du, Chengshan Yuan, Qihan Zhang, Jianrong Zhang, Shuyong Zhang. Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments[J]. University Chemistry, ;2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100 shu

Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments

Houjin Li ^1,, ,
Lin Wu ² ,
Xingwen Sun ³ ,
Yuan Zheng ⁴ ,
Zhanxiang Liu ⁵ ,
Shuanglian Cai ⁶ ,
Ying Xiong ⁷ ,
Guangao Yu ⁸ ,
Qingwen Liu ⁹ ,
Jie Han ¹⁰ ,
Xin Du ¹¹ ,
Chengshan Yuan ¹² ,
Qihan Zhang ¹³ ,
Jianrong Zhang ^2,, ,
Shuyong Zhang ^14,,

1.
School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, China;
2.
National Demonstration Center for Experimental Chemistry Education (Nanjing University), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
3.
National Demonstration Center for Experimental Chemistry (Fudan University), Department of Chemistry, Fudan University, Shanghai 200433, China;
4.
National Demonstration Center for Experimental Chemistry Education (University of Science and Technology of China), School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China;
5.
National Demonstration Center for Experimental Chemistry Education (Zhejiang University), Department of Chemistry, Zhejiang University, Hangzhou 310058, China;
6.
College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;
7.
National Demonstration Center for Experimental Chemistry Education (Wuhan University), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
8.
College of Chemistry, Central China Normal University, Wuhan 430079, China;
9.
National Demonstration Center for Experimental Chemistry Education (Jilin University), College of Chemistry, Jilin University, Changchun 130012, China;
10.
National Demonstration Center for Experimental Chemistry Education (Nankai University), College of Chemistry, Nankai University, Tianjin 300071, China;
11.
School of Chemistry, Dalian University of Technology, Dalian 116024, Liaoning Province, China;
12.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;
13.
National Demonstration Center for Experimental Chemistry Education (Peking University), College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
14.
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China

Corresponding author: Houjin Li, Jianrong Zhang, Shuyong Zhang,
Received Date: 24 August 2024
Revised Date: 13 September 2024

Chromatography experiments are a fundamental experimental skill that must be mastered by the students majoring in chemistry in universities. Thin-layer chromatography, column chromatography, and paper chromatography experiments constitute the basic content of organic chemistry experiments. However, the training content, requirements, and standards for chromatography experiments vary among different universities, and lead to disparities in teaching outcomes. This article puts forward basic operation and standard suggestions for organic chemical layer chromatography, column chromatography and paper chromatography experiments, hoping to provide references for domestic peers in conducting experimental teaching and scientific research practices.
- Organic chemistry experiment,
- Thin layer chromatography,
- Column chromatography,
- Paper chromatography,
- Operation standard
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