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Citation: Yu Dai, Xueting Sun, Haoyu Wu, Naizhu Li, Guoe Cheng, Xiaojin Zhang, Fan Xia. Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide[J]. University Chemistry, ;2025, 40(5): 351-356. doi: 10.12461/PKU.DXHX202407052 shu

Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide

Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430078, China

Corresponding author: Yu Dai, Fan Xia,
Received Date: 11 July 2024
Revised Date: 14 September 2024

In recent years, gold nanoparticles (AuNPs) have been identified as possessing nanozyme properties, enabling them to mimic the catalytic activity of natural peroxidases in the decomposition of hydrogen peroxide (H₂O₂). In this study, AuNPs were synthesized using gelatin as a reducing agent, and the Michaelis constant for the AuNP-catalyzed decomposition of hydrogen peroxide was determined using UV-Vis spectroscopy. Integrating the innovative concept of “nanozyme” into a physical chemistry kinetics experiment designed for undergraduate students not only enhances their comprehensive skills but also fosters their interest in chemical experimentation and instills a sense of national pride.
- Nanozyme,
- Gold nanoparticle,
- Hydrogen peroxide decomposition,
- Michaelis constant,
- Physical chemistry experiment
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