Citation: ZHANG Shen, GUO Yuyu. One-Pot Synthesis of Fluorescent Polyvinyl Pyrrolidone-Stabilized Cu Nanoclusters for the Determination of Quercetin[J]. Chinese Journal of Applied Chemistry, ;2020, 37(9): 1069-1075. doi: 10.11944/j.issn.1000-0518.2020.09.200045 shu

One-Pot Synthesis of Fluorescent Polyvinyl Pyrrolidone-Stabilized Cu Nanoclusters for the Determination of Quercetin

ZHANG Shen ^a ,
GUO Yuyu ^b,,

a.
Department of Chemistry, Taiyuan Normal University, Jinzhong, Shanxi 030619, China
b.
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China

Corresponding author: GUO Yuyu, guoyuyu@tyut.edu.cn
Received Date: 17 February 2020
Revised Date: 23 April 2020
Accepted Date: 3 June 2020

Fund Project: Supported by the Shanxi Provincial Applied Fundamental Research Fund Project(No.201801D121257)Shanxi Provincial Applied Fundamental Research Fund Project 201801D121257

Figures(6)

As an effective way to detect quercetin, it is important to synthesize fluorescent probe with excellent performance by a simple method. In this work, a facile one-pot approach has been developed to prepare blue-emitting Cu nanoclusters (NCs) using ascorbic acid as a reducing agent and polyvinyl pyrrolidone (PVP) as a capping agent. The PVP-stabilized PVP-Cu NCs display good dispersion, high stability and strong fluorescence. It exhibits good water solubility, excellent photostability and high stability toward high concentration of sodium chloride. The optical performance and structure of PVP-Cu NCs were characterized by ultraviolet-visible (UV-Vis) absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The characterization results show that PVP-Cu NCs have a strong symmetric emission at 429 nm with a maximum excitation at 366 nm. TEM analysis shows that the average diameter of PVP-Cu NCs is 2.0 nm. We construct a fluorescent sensor for the detection of quercetin on the basis of the quenching effect of quercetin on the fluorescence of PVP-Cu NCs. The experimental results show that the quercetin sensing system has a linear range of 0.1~0.9 μmol/L and 15~60 μmol/L with a low detection limit (S/N=3) of 0.053 μmol/L. The sensor has high sensitivity and good selectivity for the detection of quercetin, and can be applied for quercetin detection in real samples.
- copper nanoclusters,
- polyvinyl pyrrolidone,
- quercetin,
- fluorescence quenching
1. [1]
  Borska S, Chmielewska M, Wysocka T. In Vitro Effect of Quercetin on Human Gastric Carcinoma:Targeting Cancer Cells Death and MDR[J]. Food Chem Toxicol, 2012,50(9):3375-3383. doi: 10.1016/j.fct.2012.06.035
2. [2]
  Rogerio A P, Dora C L, Andrade E L. Anti-inflammatory Effect of Quercetin-loaded Microemulsion in the Airways Allergic Inflammatory Model in Mice[J]. Pharmacol Res, 2010,61(4):288-397. doi: 10.1016/j.phrs.2009.10.005
3. [3]
  Ferrer E G, Salinas M V, Correa M J. Synthesis, Characterization, Antitumoral and Osteogenic Activities of Quercetin Vanadyl(IV) Complexes[J]. J Biol Inorg Chem, 2006,11(6):791-801. doi: 10.1007/s00775-006-0122-9
4. [4]
  Kim Y H, Lee D H, Jeong J H. Quercetin Augments TRAIL-Induced Apoptotic Death:Involvement of the ERK Signal Transduction Pathway[J]. Biochem Pharmacol, 2008,75(10):1946-1958. doi: 10.1016/j.bcp.2008.02.016
5. [5]
  Gil J J, Langner E, Wertel I. Temozolomide, Quercetin and Cell Death in the MOGGCCM Astrocytoma Cell Line[J]. Chem Biol Interact, 2010,188(1):190-203. doi: 10.1016/j.cbi.2010.07.015
6. [6]
  Pejic N, Kuntic V, Vujic Z. Direct Spectrophotometric Determination of Quercetin in the Presence of Ascorbic Acid[J]. Il Farmaco, 2004,59(1):21-24. doi: 10.1016/j.farmac.2003.07.013
7. [7]
  Nugroho A, Lim S C, Lee C M. Simultaneous Quantitative Determination and Validation of Quercetin Glycosides with Peroxynitrite-Scavenging Effects from Saussurea grandifolia[J]. J Pharm Biomed Anal, 2001,61(5):247-251.
8. [8]
  Yu J B, Jin H, Gui R J. A Facile Strategy for Ratiometric Electrochemical Sensing of Quercetin in Electrolyte Solution Directly Using Bare Glassy Carbon Electrode[J]. J Electroanal Chem, 2017,795:97-102. doi: 10.1016/j.jelechem.2017.04.053
9. [9]
  Wu D D, Chen Z. ZnS Quantum Dots-Based Fluorescence Spectroscopic Technique for the Detection of Quercetin[J]. Luminescence, 2014,29(4):307-313. doi: 10.1002/bio.2545
10. [10]
  Dwiecki K, Kwiatkowska P, Siger A. Determination of Quercetin in Onion (Allium cepa) Using β-Cyclodextrin-Coated CdSe/ZnS Quantum Dot-Based Fluorescence Spectroscopic Technique[J]. Int J Food Sci Tech, 2015,50(6):1366-1373. doi: 10.1111/ijfs.12772
11. [11]
  Gao Y F, Jin X, Kong F Y. One-pot Green and Simple Synthesis of Actinian Nickel-Doped Carbon Nanoflowers for Ultrasensitive Sensing of Quercetin[J]. Analyst, 2019,144(24):7283-7289. doi: 10.1039/C9AN01907C
12. [12]
  Aparna R S, Devi J S A, Anjana R R. Reversible Fluorescence Modulation of BSA Stabilised Copper Nanoclusters for the Selective Detection of Protamine and Heparin[J]. Analyst, 2019,144(5):1799-1808. doi: 10.1039/C8AN01703D
13. [13]
  Aparna R S, Syamchand S S, George S. Tannic Acid Stabilised Copper Nanocluster Developed Through Microwave Mediated Synthesis as a Fluorescent Probe for the Turn On Detection of Dopamine[J]. J Clust Sci, 2017,28(4):2223-2238. doi: 10.1007/s10876-017-1221-1
14. [14]
  Bhamore J R, Jha S, Mungara A K. One-step Green Synthetic Approach for the Preparation of Multicolor Emittingcopper Nanoclusters and Their Applications in Chemical Species Sensing and Bioimaging[J]. Biosens Bioelectron, 2016,80:243-248. doi: 10.1016/j.bios.2016.01.066
15. [15]
  Ai L, Jiang W R, Liu Z Y. Engineering a Red Emission of Copper Nanocluster Self-assembly Architectures by Employing Aromatic Thiols as Capping Ligands[J]. Nanoscale, 2017,9:12618-12627. doi: 10.1039/C7NR03985A
16. [16]
  Basu K, Gayen K, Mitra T. Different Color Emissive Copper Nanoclusters and Cancer Cell Imaging[J]. ChemNanoMat, 2017,3:808-814. doi: 10.1002/cnma.201700162
17. [17]
  Bagheri H, Afkhami A, Khoshsafar H. Protein Capped Cu Nanoclusters-SWCNT Nanocomposite as a Novel Candidate of High Performance Platform for Organophosphates Enzymeless Biosensor[J]. Biosens Bioelectron, 2017,89:829-836. doi: 10.1016/j.bios.2016.10.003
18. [18]
  Baig M M F, Chen C T, Chen Y C. Photoluminescence Determination of Aluminum Using Glutathione-Capped Gold Nanoclusters[J]. Anal Lett, 2016,49(14):2246-2258. doi: 10.1080/00032719.2016.1143477
19. [19]
  Chen P C, Ma J Y, Chen L Y. Photoluminescent AuCu Bimetallic Nanoclusters as pH Sensors and Catalysts[J]. Nanoscale, 2014,6(7):3503-3507. doi: 10.1039/c3nr06123j
20. [20]
  Lu D T, Chen Z, Li Y F. Determination of Mercury(II) by Fluorescence Using Deoxyribonucleic Acid Stabilized Silver Nanoclusters[J]. Anal Lett, 2014,48(2):281-290.
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