Citation: ZHOU Zhuoyu, SUI Xiaochen, XIA Yongmei. Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells[J]. Chinese Journal of Applied Chemistry, ;2020, 37(7): 785-792. doi: 10.11944/j.issn.1000-0518.2020.07.200020 shu

Enzymatic Synthesis of Rubusoside and Its Inhibition on Liver and Gastrointestinal Cells

ZHOU Zhuoyu ^a,b ,
SUI Xiaochen ^a,b ,
XIA Yongmei ^a,b,,

a.
School of Chemical and Materials Engineering Jiangnan University, Wuxi, Jiangsu 214000, China
b.
State Key Laboratory of Food Science and Technology, Wuxi, Jiangsu 214000, China

Corresponding author: XIA Yongmei, ymxia@jiangnan.edu.cn
Received Date: 16 January 2020
Revised Date: 30 March 2020
Accepted Date: 11 May 2020

Fund Project: the National Industry Technology and Engineering Double First-Class Discipline Program LITE2018-03the National Natural Science Foundation of Chin 31371837the National Natural Science Foundation of Chin 31772017Supported by the National Natural Science Foundation of China(No.31772017, No.31371837.), the National Industry Technology and Engineering Double First-Class Discipline Program(No.LITE2018-03)

Figures(7)

Rubusoside is a rare natural sweetener. In this experiment, a β-glucosidase from Aspergillus niger was screened from some glycosidases, which was used to proficiently produce rubusoside from stevioside. The optimum rubusoside yield and the stevioside conversion reach 90.4% and 98.8% in 12 h, respectively. Subsequently, the cytotoxicity of rubusoside on human gastrointestinal and liver cells was investigated, which includes 3 normal and 11 cancer cell lines. At a mass concentration of 250 μg/mL, rubusoside is not toxic to the normal cells, and its inhibition rate of rubusoside on BEL-7404 is 30% of that 5-fluorouracil proceeded.
- rubusoside,
- steviolside,
- β-glucosidase,
- enzyme catalysis,
- anti-cancer
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