Citation: XU Liping, LIU Qingshi, DONG Zhichen, GUO Xingjia, DONG Wei. Simple, Fast and Accurate Detection of Ciprofloxacin Based on Fluorescence Enhancement of Nitrogen-Doped Carbon Dots[J]. Chinese Journal of Applied Chemistry, ;2020, 37(7): 830-838. doi: 10.11944/j.issn.1000-0518.2020.07.190318 shu

Simple, Fast and Accurate Detection of Ciprofloxacin Based on Fluorescence Enhancement of Nitrogen-Doped Carbon Dots

a.
College of Chemistry, Liaoning University, Shenyang 110036, China
b.
Department of Chemistry, Shenyang Medical College, Shenyang 110034, China

Corresponding author: DONG Wei, dongwei5873@126.com
Received Date: 26 November 2019
Revised Date: 31 March 2020
Accepted Date: 28 April 2020

Fund Project: Supported by the Natural Science Foundation of Liaoning Province(No.20180550105)the Natural Science Foundation of Liaoning Province 20180550105

Figures(7)

Nitrogen doped fluorescence carbon dots (N-CDs) were successfully synthesized by a facile one step solid phase pyrolysis treatment using malic acid as the carbon source and ammonium phosphate as the nitrogen source. The fluorescence quantum yield of the obtained N-CDs reaches 20.7% and the N-CDs have approximately spherical morphology with an average diameter of 3.3 nm. The as prepared N-CDs were used as the fluorescent probe to detect ciprofloxacin (CIP) based on the enhancement of N-CDs fluorescence upon adding CIP. The optimal experimental mass conditions are 7.5 μg/mL of N-CDs, pH=5.91, and 5 min of incubation time for the detection of CIP. The enhancement of N-CDs fluorescence exhibits a good linear relationship with the concentration of CIP ranged from 0.39 μmol/L to 40.0 μmol/L under the optimal conditions. The linear regression equation is ΔF=1.61×10⁷[CIP]-3.28 with a correlation coefficient R²=0.994. The detection limit and relative standard deviation (n=5) are estimated to be 0.12 mol/L and 4.2%, respectively. The interference experiments indicate that potential coexistence substances (except copper ions) have ignorable effects on the detection of CIP, and the interference of copper ions could be masked with 4% ammonium oxalate solution. Finally, the proposed sensor was successfully applied to analyze real samples with satisfactory results.
- nitrogen doped carbon dots,
- ciprofloxacin,
- fluorescence probe,
- fluorescence enhancement
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