Citation: XIE Xingqin. Interaction Mechanism Between Thiourea Aryl Iridium Anticancer Complex and Bovine Serum Albumin[J]. Chinese Journal of Applied Chemistry, ;2020, 37(5): 604-610. doi: 10.11944/j.issn.1000-0518.2020.05.190303 shu

Interaction Mechanism Between Thiourea Aryl Iridium Anticancer Complex and Bovine Serum Albumin

XIE Xingqin ^,

Department of Metallurgy and Resource Engineering, Nanning Campus, Guilin University of Technology, Chongzuo, Guangxi 532100, China

Corresponding author: XIE Xingqin, 522914369@qq.com
Received Date: 14 November 2019
Revised Date: 9 January 2020
Accepted Date: 20 February 2020

Fund Project: the Guangxi University Young Teachers Basic Ability Promotion Project 2019KY0288Supported by the Guangxi University Young Teachers Basic Ability Promotion Project(No.2019KY0288)

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In this paper, the interaction between thiourea aryl iridium and bovine serum albumin (BSA) was studied by ultraviolet-visible spectroscopy (UV-Vis) and fluorescence spectroscopy under physiological conditions. The mechanism of action was determined, the type of binding force was discussed, and the activation energy of the reaction of thiourea aryl iridium anticancer compound with bovine serum albumin was calculated. The experimental results show that m-methoxybenzaldehyde 4-phenyl-3-thiourea aryl iridium (TSC-Ir-6) complex has a quenching effect on endogenous fluorescence of bovine serum albumin, and the quenching type is static quenching. Through the fixed Stern-Volmer equations and apparent binding constant, the thermodynamic parameters calculated by the formula, it is concluded that the combination of TSC-Ir-6 and BSA is a spontaneous process (ΔG < 0), and the interaction forces are hydrogen bonding and van der Waals force. The number of binding sites is about one. With the presence of coexisting ions, the binding constant between TSC-Ir-6 and BSA is significantly increased, the binding force is stronger, and the retention time of TSC-Ir-6 in plasma is improved, which may lead to a better therapeutic effect.
- thiourea aryl iridium complex,
- bovine serum albumin,
- ultraviolet-visible spectroscopy,
- fluorescent spectrometry,
- interaction
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