Citation: YANG Jianping, SU Tingting, QIU Xiaozhen, ZHANG Hanjie, WANG Yali, YIN wei. Screening of Hypoglycemic Constituents in Chinese Herbal Medicine Based on Electrochemical Amperometric Detection[J]. Chinese Journal of Applied Chemistry, ;2018, 35(7): 842-848. doi: 10.11944/j.issn.1000-0518.2018.07.170312 shu

Screening of Hypoglycemic Constituents in Chinese Herbal Medicine Based on Electrochemical Amperometric Detection

Engineering Technology Development Center of Advanced Materials & Energy Saving and Emission Reduction in Guangdong Colleges and Universities, Department of Chemistry, Guangdong University of Education, Guangzhou 510303, China

Corresponding author: YANG Jianping, yangjianping@gdei.edu.cn
Received Date: 1 September 2017
Revised Date: 25 October 2017
Accepted Date: 29 November 2017

Fund Project: the Natural Science Foundation of Guangdong Province 2015A030310202Supported by the Natural Science Foundation of Guangdong Province(No.2015A030310202), Higher Education Reformation Projects(No.2016ybzz08), Innovative Training Program For College Students(No.201714278005, No.201714278075), Project of Engineering Technology Development Center of Advanced Materials & Energy Saving and Emission Reduction in Guangdong Colleges and Universities(No.2016GCZX007)Higher Education Reformation Projects 2016ybzz08Innovative Training Program For College Students 201714278075Project of Engineering Technology Development Center of Advanced Materials & Energy Saving and Emission Reduction in Guangdong Colleges and Universities 2016GCZX007Innovative Training Program For College Students 201714278005

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A novel method for the extraction, isolation, identification and determination of the active component of Chinese medicine and its inhibitory activity on glucoamylase in a simple and efficient way was developed.The active ingredient baicalin was extracted from Scutellaria baicalensis using green nontoxic water-extracting and acid-depositing method.It was identified qualitatively by thin layer chromatography and the content was determined by ultraviolet spectrophotometry.An effective electrochemical glucose sensor was fabricated and a new method of characterizing hypoglycemic activity was developed based on electrochemical amperometric detection.Acarbose(clinical antidiabetic drug) was further used to demonstrate the reliability and stability of determining the inhibitory activity of glucoamylase by electrochemical amperometry.The results show that the baicalin has a good inhibitory activity on glucoamylase with a half maximal inhibitory concentration(IC₅₀) at 0.056 g/L.This study provides a novel method for the extraction and isolation of hypoglycemic components from natural medicines.
- baicalin,
- glucoamylase,
- electrochemical method,
- inhibitory activity
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