Citation: WANG Yue, CAI Chen, LIU Huiling, SUN Li, LI Yanchao, MENG Zhaohong. Quantitative Analysis of Cephalosporin C in Soil by High Performance Liquid Chromatography with Ultrasound-assisted Extraction and Solid-Phase Extraction[J]. Chinese Journal of Applied Chemistry, ;2018, 35(6): 722-728. doi: 10.11944/j.issn.1000-0518.2018.06.170245 shu

Quantitative Analysis of Cephalosporin C in Soil by High Performance Liquid Chromatography with Ultrasound-assisted Extraction and Solid-Phase Extraction

WANG Yue ^a ,
CAI Chen ^b ,
LIU Huiling ^b ,
SUN Li ^c ,
LI Yanchao ^d ,
MENG Zhaohong ^a,,

a.
College of Geographic Science, Harbin Normal University, Harbin 150025, China
b.
State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin 150090, China
c.
College of Geographic Science, Harbin Normal University, Harbin 150025, China
d.
Electronic Engineering College, Heilongjang University, Harbin 150080, China

Corresponding author: MENG Zhaohong, mengzhaohong1973@163.com
Received Date: 12 July 2017
Revised Date: 6 September 2017
Accepted Date: 9 November 2017

Fund Project: Supported by the National Natural Science Foundation of China(No.41401589), the Natural Science Foundation of Heilongjiang Province, Special Research Funds for the Universities of Heilongjiang Province(No.HDRCCX-2016Z10)Special Research Funds for the Universities of Heilongjiang Province HDRCCX-2016Z10the National Natural Science Foundation of China 41401589

Figures(3)

A rapid and simple method based on ultrasound-assisted extraction, solid-phase extraction followed by high performance liquid chromatography(UAE-SPE-HPLC) was proposed for the determination of cephalosporin C(CPC) in soil. The optimum pre-treatment parameters for the soil samples contaminated with CPC are as follow:ultra-pure water as extraction solvent for CPC, SPE as solid phase extraction cartridge, 5.0 mL 10% methanol as rinse solvent, 2.0 mL 5% formic acid/methanol solution(volume ratio 50:50) as eluent. The results show that when 0.1% formic acid and methanol(95:5), 30℃ column temperature, and 254 nm detection wavelength were applied during the isocratic elution process, HPLC assay offeres a good potential for the sensitive and selective determination of CPC. The recovery rates for seven different treatments are 77.9%~98.8%, and the precision is 5.0%~6.3%(n=5). The limit of determination(LOD) and the limit of quantification(LOQ) in soil are 340.4 μg/kg and 1126.8 μg/kg, respectively. At the same time, this method was used to detect the positive soil near the pharmaceutical factory in Xinjiang. The experimental results of different batches show detected(below LOQ), 1532.1 μg/kg and detected(below LOQ), respectively. In conclusion, UAE-SPE-HPLC is simple and rapid. Its accuracy and precision are in line with the quality control requirements. It is suitable for the detection of trace CPC in soil.
- cephalosporin C,
- soil,
- ultrasound-assisted extraction,
- solid-phase extraction,
- high performance liquid chromatography
1. [1]
  XUE Yu, CHENG Yuying. New Development of Cephalosporin Antibiotics[J]. Chinese J Antibiot, 2011,36(2):86-92.
2. [2]
  LI Zaixing, TIAN Baokuo, ZUO Jian'e. Progress in Treatment and Disposal Technology of Antibioyic Bacterial Residues[J]. Environ Eng, 2012,30(2):72-75.
3. [3]
  DUAN Ziheng, LIU Huiling, LIU Qingliang. Effects of Penicillin Biowaste Fertilizeronsoil Microorganisms and Enzyme Activities in Tomato Rhizophere[J]. Envrion Eng, 2015,33(11):115-119.
4. [4]
  NIE Shengwei, LI Xiangdong, ZHANG Yuting. Effects of Different Edible Fungus Residue Fertilizer Application Amounts on Wheat Yield and Yield Components[J]. J He'nan Agric Sci, 2015,44(6):76-80.
5. [5]
  Elshaboury S R, Saleh G A, Mohamed F A. Analysis of Cephalosporin Antibiotics[J]. J Pharm Biomed Anal, 2007,45(1):1-19. doi: 10.1016/j.jpba.2007.06.002
6. [6]
  LIU Shijuan. Eatablishment on HPLC-MS/MS Detection Methods for Multi-Cephalosporins in Muscle Tissues[D]. Anhui: Anhui Agricultural Unversity, 2012(in Chinese).
7. [7]
  WANG Yuqin, LIU Hua, HAO Hongyuan. Analysis of 3 Peptides Antibiotics in Pork by Solid Phase Extraction and Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese J Anal Lab, 2017(1):73-77.
8. [8]
  Molina M P, Althaus R L, Molina A. Antimicrobial Agent detection in Ewes' Milk by the Microbial Inhibitor Test Brilliant Black Reduction Test-BRT AiM^®[J]. Int Dairy J, 2003,13(10):821-826. doi: 10.1016/S0958-6946(03)00107-9
9. [9]
  Montero A, Althaus R L, Molina A. Detection of Antimicrobial Agents by a Specific Microbiological Method (Eclipse100^®; ) for Ewe Milk[J]. Small Ruminant Res, 2005,57(2):229-237.
10. [10]
  Huth S P, Warholic P S, Devou J M. Parallux Beta-lactam:A Capillary-based Fluorescent Immunoassay for the Determination of Penicillin-G, Ampicillin, Amoxicillin, Cloxacillin, Cephapirin, and Ceftiofur in Bovine Milk[J]. J AOAC Int, 2002,85(2):355-364.
11. [11]
  Kress C, Seidler C, Kerp B. Experiences with an Identification and Quantification Program for Inhibitor-positive Milk Samples[J]. Anal Chim Acta, 2007,586(1/2):275-279.
12. [12]
  XIE Huiling. Research on Immunocharomatography Strips and ELISA Kits for Determination[D]. Jiangsu: Jiangnan University, 2009(in Chinese).
13. [13]
  Kurittu J, Lönnberg S, Virta M. A Group-specific Microbiological Test for the Detection of Tetracycline Residues in Raw Milk[J]. J Agric Food Chem, 2000,48(8):3372-3377. doi: 10.1021/jf9911794
14. [14]
  Chan P H, Liu H B, Chen Y W. Rational Design of a Novel Fluorescent Biosensor for Beta-lactam Antibiotics from a Class A Beta-lactamase[J]. J Am Chem Soc, 2004,126(13):4074-4075. doi: 10.1021/ja038409m
15. [15]
  LIN Xiaoxu, HE Shiyan, YE Yongsheng. High Performance Liquid Chromatographic Fuorescence Detection Method Also Analyzes Four Quinolones Antibiotics in the Biogas[J]. Chem Enterprise Manage, 2017,1(2)77.
16. [16]
  Beconi-Barker M G, Roof R D, Millerioux L. Determination of Ceftiofur and Its Desfuroylceftiofur-related Metabolites in Swine Tissues by High-performance Liquid Chromatography[J]. J Chromatogr B Biomed Appl, 1995,673(2):231-244. doi: 10.1016/0378-4347(95)00258-1
17. [17]
  Keever J, Voyksner R D, Tyczkowska K L. Quantitative Determination of Ceftiofur in Milk by Liquid Chromatography-Electrospray Mass Spectrometry[J]. J Chromatogr A, 1998,794(1/2):57-62.
18. [18]
  YIN Xiaoyan, LIU Yanqin, CAO Hong, et al. A Method for Determination of 12 Kinds of Beta-lactamide in Milk by Liquid Chromatography-Seriesion Trap Mass Spectrometry[C]//National Organic Mass Spectrum Academic Exchange Meeting, Chengdu: China Analysis and Testing Institute, 2007, 26: 272-274(in Chinese).
19. [19]
  GU Peiqiao, MEI Guangming, ZHANG Xiaojun. Determination of 8 Kinds of Cephalosporins in Aquatic Products by Multi-walled Carbon Nanotubes Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Mass Spectrometry[J]. Chinese J Anal Chem, 2017,45(3):381-388. doi: 10.11895/j.issn.0253-3820.160633
20. [20]
  MA Shanshan, LIU Yan, YU Ran. Determination of sodium Penicillin in Soil Through Accelerated Solvent Extraction and Solid Phase Extraction followed by High Performance Liquid Chromatography(ASE-SPE-HPLC)[J]. Environ Chem, 2014,33(11):1978-1985. doi: 10.7524/j.issn.0254-6108.2014.11.002
21. [21]
  Suhren G, Knappstein K. Detection of Cefquinome in Milk by Liquid Chromatography and Screening Methods[J]. Anal Chim Acta, 2003,483(483):363-372.
22. [22]
  Shin H Y, Lee J Y, Jung Y R. Stimulation of Cephalosporin C Production in Acremonium Chrysogenum M35 by Glycerol[J]. Bioresour Technol, 2010,101(12):4549-4553. doi: 10.1016/j.biortech.2010.01.095
23. [23]
  Shin H Y, Lee J Y, Park C. Utilization of Glycerol as Cysteine and Carbon Sources for Cephalosporin C Production by Acremonium Chrysogenum M35 in Methionine-unsupplemented Culture[J]. J Biotechnol, 2011,151(4):363-368. doi: 10.1016/j.jbiotec.2010.12.021
24. [24]
  Duan S, Yuan G, Zhao Y. Enhanced Cephalosporin C Production with a Combinational Ammonium Sulfate and DO-Stat Based Soybean Oil Feeding Strategy[J]. Biochem Eng J, 2012,61(4):1-10.
25. [25]
  Benito-Pena E, Urraca J B M. Quantitative Determination of Penicillin V and Amoxicillin in Feed Samples by Pressurised Liquid Extraction and Liquid Chromatography with Ultraviolet Detection[J]. J Pharmceut Biomed, 2009,49(2):289-294. doi: 10.1016/j.jpba.2008.11.016
26. [26]
  Anastassiades M, Lehotay S J, Stajnbaher D. Fast and Easy Multiresidue Method Employing Acetonitrile Extraction/partitioning and "Dispersive Solid-Phase Extraction" for the Determination of Pesticide Residues in Produce[J]. J Aoac Int, 2003,86(2):412-431.
27. [27]
  Lara F J, Qlmo-lruela M D, Cruces-Blanco C. Advances in the Determination of β-Lactam Antibiotics by Lliquid Chromatography[J]. Trac-Trend Anal Chem, 2012,38:52-66. doi: 10.1016/j.trac.2012.03.020
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