Citation: CAI Qihong, CHEN Xiaoping, LU Jiaxing, CHEN Dengxiu. Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry[J]. Chinese Journal of Applied Chemistry, ;2018, 35(5): 609-612. doi: 10.11944/j.issn.1000-0518.2018.05.170210 shu

Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry

Key Laboratory of Pharmaceutical Analysis and Laboratory Medicine of Fujian Province University, Pharmaceutical and Medical Technology College, Putian University, Putian, Fujian 351100, China

Corresponding author: CAI Qihong, cqh118@163.com
Received Date: 15 June 2017
Revised Date: 7 August 2017
Accepted Date: 4 September 2017

Fund Project: Supported by the Leading Project of Fujian Provincial Science and Technology Program(No.2015Y0030), the Scientific Research Project of the Education Departerment of Fujian Province(No.JK2014045), the Science and Technology Project of Putian City[No.2013S01(2)], the Scientific Research Project of Putian University(No.2015088)the Scientific Research Project of the Education Departerment of Fujian Province JK2014045the Science and Technology Project of Putian City 2013S01(2)the Scientific Research Project of Putian University 2015088the Leading Project of Fujian Provincial Science and Technology Program 2015Y0030

Figures(3)

Based on the similarity of the fluorescence properties of three estrogens, a new fluorescence method for direct determination of the total amount of three estrogens(estradiol, estriol, ethinyloestradiol) in milk powder was developed. Taking estriol as the standard reference material, the standard curve method was adopted. The fluorescence intensity of estriol has a linear relationship with the concentration in the range of 1.00~2000 nmol/L and the detection limit of 0.16 nmol/L. The recovery is in the range of 89.11%~98.49%. This method is simple, rapid, highly accurate and sensitive and has been successfully applied to determine the total estrogens in milk powder with satisfactory results.
- estrogens,
- fluorescence spectrometry,
- milk powder,
- estradiol,
- estriol,
- ethinyloestradiol
1. [1]
  Armstrong B, Doll R. Environmental Factors and Cancer Incidenceandmortality in Different Countries, with Special Reference to Dietary Practices[J]. Int J Cancer, 1975,15(4):617-631. doi: 10.1002/(ISSN)1097-0215
2. [2]
  Rose D P, Boyar A P, Wynder E L. International Comparisons of Mortality Rates for Cancer of the Breast, Ovary, Prostate, and Colon, and Per Capita Food Consumption[J]. Cancer, 1986,58(11):2363-2371. doi: 10.1002/(ISSN)1097-0142
3. [3]
  Qin L Q, Wang P Y, Kaneko T. Estrogen:One of the Risk Factors in Milk for Prostate Cancer[J]. Med Hypoth, 2004,62(1):133-142. doi: 10.1016/S0306-9877(03)00295-0
4. [4]
  YUAN Lijun, XU Zhuangjian, XU Xiangsheng. The Estrogen in Cow's Milk and Its Safety[J]. Int J Inter Med, 2007,34(4):712-714.
5. [5]
  Kaipparettu B A, Malik S, Konduri S D. Estrogenmediated Downregulation of CD24 in Breast Cancer Cells[J]. Int J Cancer, 2008,123(1):66-72. doi: 10.1002/(ISSN)1097-0215
6. [6]
  Wang L, Yan H Y, Yang C L. Synthesis of Mimic Molecularly Imprinted Ordered Mesoporous Silica Adsorbent by Thermally Reversible Semicovalent Approach for Pipette-Tip Solid-Phase Extraction-Liquid Chromatography Fluorescence Determination of Estradiol in Milk[J]. J Chromatogr A, 2016,1456(1):58-67.
7. [7]
  Xiao X H, Yin Y, Hu Y L. Microwave-Assisted Extraction Coupled with Single Drop Microextraction and High-Performance Column Liquid Chromatography for the Determination of Trace Estrogen Adulterants in Soybean Isoflavone Dietary Supplements[J]. J AOAC Int, 2010,93(3):849-854.
8. [8]
  XIAO Fugang, GUO Weiyun, LIU Yan. Determination of Estradiol in Milk Powder by HPLC[J]. Food Res Dev, 2011,32(8):81-83.
9. [9]
  ZHANG Hongying, ZHANG Hong, WANG Shuhui. Determination of Environmental Estrogens in Milk by Gas Chromatography-Mass Spectrometry[J]. Chinese J Health Lab Technol, 2009,19(8):1713-1715.
10. [10]
  LIU Yonglin, TIAN Chengxin, YAN Tianwen. Determination of β-Estradiol in Bovine Colostrum by Gas Chromatography-Mass Spectrometry[J]. China Dairy Ind, 2014,42(7)53/54/55, 59.
11. [11]
  Tso J, Aga D S. A Systematic Investigation to Optimize Simultaneous Extraction and liquid Chromatography Tandem Mass Spectrometry Analysis of Estrogens and Their Conjugated Metabolites in Milk[J]. J Chromatogr A, 2010,1217(29):4784-4795. doi: 10.1016/j.chroma.2010.05.024
12. [12]
  Yang Y, Shao B, Zhang J. Determination of the Residues of 50 Anabolic Hormones in Muscle, Milk and Liver by Very-High-Pressure Liquid Chromatodraphy-Electrospray Ionization Tandem Mass Spectrometry[J]. J Chromatogr B, 2009,877(5/6):489-496.
1. [1]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280
2. [2]
  Xinhao Yan , Guoliang Hu , Ruixi Chen , Hongyu Liu , Qizhi Yao , Jiao Li , Lingling Li . Polyethylene Glycol-Ammonium Sulfate-Nitroso R Salt System for the Separation of Cobalt (II). University Chemistry, 2024, 39(6): 287-294. doi: 10.3866/PKU.DXHX202310073
3. [3]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
4. [4]
  Yiling Wu , Peiyao Jin , Shenyue Tian , Ji Zhang . The Star of Sugar Substitutes: An Interview of Erythritol. University Chemistry, 2024, 39(9): 22-27. doi: 10.12461/PKU.DXHX202404034
5. [5]
  Aili Feng , Xin Lu , Peng Liu , Dongju Zhang . Computational Chemistry Study of Acid-Catalyzed Esterification Reactions between Carboxylic Acids and Alcohols. University Chemistry, 2025, 40(3): 92-99. doi: 10.12461/PKU.DXHX202405072
6. [6]
  Zhanggui DUAN , Yi PEI , Shanshan ZHENG , Zhaoyang WANG , Yongguang WANG , Junjie WANG , Yang HU , Chunxin LÜ , Wei ZHONG . Preparation of UiO-66-NH₂ supported copper catalyst and its catalytic activity on alcohol oxidation. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 496-506. doi: 10.11862/CJIC.20230317
7. [7]
  Yifeng TAN , Ping CAO , Kai MA , Jingtong LI , Yuheng WANG . Synthesis of pentaerythritol tetra(2-ethylthylhexoate) catalyzed by h-MoO₃/SiO₂. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2155-2162. doi: 10.11862/CJIC.20240147
8. [8]
  Zhaoxuan ZHU , Lixin WANG , Xiaoning TANG , Long LI , Yan SHI , Jiaojing SHAO . Application of poly(vinyl alcohol) conductive hydrogel electrolytes in zinc ion batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 893-902. doi: 10.11862/CJIC.20240368
9. [9]
  Mi Wen , Baoshuo Jia , Yongqi Chai , Tong Wang , Jianbo Liu , Hailong Wu . Improvement of Fluorescence Quantitative Analysis Experiment: Simultaneous Determination of Rhodamine 6G and Rhodamine 123 in Food Using Chemometrics-Assisted Three-Dimensional Fluorescence Method. University Chemistry, 2025, 40(4): 390-398. doi: 10.12461/PKU.DXHX202405147
10. [10]
  Ronghao Zhao , Yifan Liang , Mengyao Shi , Rongxiu Zhu , Dongju Zhang . Investigation into the Mechanism and Migratory Aptitude of Typical Pinacol Rearrangement Reactions: A Research-Oriented Computational Chemistry Experiment. University Chemistry, 2024, 39(4): 305-313. doi: 10.3866/PKU.DXHX202309101
11. [11]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
12. [12]
  Yuan Zheng , Quan Lan , Zhenggen Zha , Lingling Li , Jun Jiang , Pingping Zhu . Teaching Reform of Organic Synthesis Experiments by Introducing Reverse Thinking and Design Concepts: Taking the Synthesis of Cinnamic Acid Based on Retrosynthetic Analysis as an Example. University Chemistry, 2024, 39(6): 207-213. doi: 10.3866/PKU.DXHX202310065
13. [13]
  Jie ZHAO , Sen LIU , Qikang YIN , Xiaoqing LU , Zhaojie WANG . Theoretical calculation of selective adsorption and separation of CO₂ by alkali metal modified naphthalene/naphthalenediyne. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 515-522. doi: 10.11862/CJIC.20230385
14. [14]
  Liyang ZHANG , Dongdong YANG , Ning LI , Yuanyu YANG , Qi MA . Crystal structures, luminescent properties and Hirshfeld surface analyses of three cadmium(Ⅱ) complexes based on 2-(3-(pyridin-2-yl)-1H-pyrazol-1-yl)benzoate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1943-1952. doi: 10.11862/CJIC.20240079
15. [15]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
16. [16]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
17. [17]
  Zhaoyang WANG , Chun YANG , Yaoyao Song , Na HAN , Xiaomeng LIU , Qinglun WANG . Lanthanide(Ⅲ) complexes derived from 4′-(2-pyridyl)-2, 2′∶6′, 2″-terpyridine: Crystal structures, fluorescent and magnetic properties. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1442-1451. doi: 10.11862/CJIC.20240114
18. [18]
  Liuyun Chen , Wenju Wang , Tairong Lu , Xuan Luo , Xinling Xie , Kelin Huang , Shanli Qin , Tongming Su , Zuzeng Qin , Hongbing Ji . 软模板法诱导Cu/Al₂O₃深孔道结构促进等离子催化CO₂加氢制二甲醚. Acta Physico-Chimica Sinica, 2025, 41(6): 100054-. doi: 10.1016/j.actphy.2025.100054
19. [19]
  Tingting Yu , Si Chen , Lianglong Sun , Tongtong Shi , Kai Sun , Xin Wang . Comprehensive Experimental Design for the Photochemical Synthesis, Analysis, and Characterization of Difluoropyrroles. University Chemistry, 2024, 39(11): 196-203. doi: 10.3866/PKU.DXHX202401022
20. [20]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(370)
HTML views(63)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142