Citation: LIANG Yuejian, LAN Yuting, YANG Lulu, KONG Fanbo, LI Juan, SUN Yue. Preparation and Properties of Hemoglobin Imprinted Polymers on Three-Dimensional Nanodendrites[J]. Chinese Journal of Applied Chemistry, ;2017, 34(7): 839-846. doi: 10.11944/j.issn.1000-0518.2017.07.160465 shu

Preparation and Properties of Hemoglobin Imprinted Polymers on Three-Dimensional Nanodendrites

School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, Liaoning 116029, China

Corresponding author: SUN Yue, yuesun@lnnu.edu.cn
Received Date: 17 November 2016
Revised Date: 23 December 2016
Accepted Date: 20 January 2017

Fund Project: Project of innovation and Entrepreneurship Program for College Students in Liaoning Province 201510165027Project of Key Laboratory of Liaoning Provincial Department of Education L201683656the National Natural Science Foundation of China 21304041

Figures(5)

For sensitive bioassay of hemoglobin(Hb), we synthesized Hb imprinted polymers(PIPs) on the surface of 3D Au/Cu nanodendrites prepared on Au electrode. The 3D Au/Cu nanodendrites were fabricated by electrodeposition performed by chronoamperometry at -0.9 V(vs.SCE) for 400 s in an aqueous electrolyte containing CuSO₄·5H₂O(0.1 mol/L) and HAuCl₄·4H₂O(0.025 mol/L). The PIPs was prepared with acrylamide as functional monomer, N, N'-methylenebisacrylamide and Hb as crosslinking agent and the template, respectively. The electrode modified with PIPs was examined by cyclic voltammetry(CV) and scanning electron microscope(SEM). Assays of Hb by differential pulse voltammetry(DPV) with PIPs modified electrode show broader linear range and lower detection limit for Hb determination compared to those similar sensors based on PIPs. The linear range is 1.0×10^-14~1.0×10^-1 mg/L with a detection limit at 1.9×10^-15 mg/L(S/N=3). These results indicate that the 3D Au/Cu nanodendrites greatly improve electron transfer ability of electrodes, and also improve the detection range of electrodes modified with PIPs.
- three-dimensional nanodendrites,
- hemoglobin,
- protein imprinted polymers
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Preparation and Properties of Hemoglobin Imprinted Polymers on Three-Dimensional Nanodendrites

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com