Citation: ZHENG Yan, LIU Shu, SONG Feng-Rui, LIU Zhi-Qiang. Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ_25-35 Induced PC12 Cells[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1724-1731. doi: 10.11895/j.issn.0253-3820.181318 shu

Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ_25-35 Induced PC12 Cells

ZHENG Yan ^1,2, ,
LIU Shu ¹ ,
SONG Feng-Rui ¹ ,
LIU Zhi-Qiang ^1,3

¹ National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry & Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
² University of Chinese Academy of Sciences, Beijing 100049, China;
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 11 May 2018
Revised Date: 15 August 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81530094).

The protective effect of Ding-Zhi-Xiao-Wan (DZXW) on Aβ_25-35 induced PC12 cells was investigated from two aspects of cell apoptosis and oxidative stress. And the formula was decomposed into single herbs and three herbs to clarify the mechanisms of action, compatibility mechanisms and main active ingredients of DZXW. The viability of PC12 cells was evaluated by MTT assay and lactate dehydrogenease content, ginseng and poria cocos played the key roles in enhancing cell viability. Compared with the model group, it could improve the cell viability about 30%. The protection mechanism of DZXW against Aβ_25-35 induced PC12 cells injury on oxidative stress and cell apoptosis pathways were studied by flow cytometry, mitochondrial membrane potential (MMP), content of malondialdehyde (MDA) and glutathione (GSH) for the further research. Ginseng and poria could significantly inhibit the cell apoptosis, and also decreased MDA content to reduce the membrane lipid peroxidation. The effect of polygala radix (PR) and acorus tatarinowii rhizoma (ATR) could improve the content of GSH in PC12 cells to inhibit oxidative stress injuries, and the activities of ginseng and poria were improved after the compatibility with PR and ATR. Overall speaking, the compatibility of DZXW formula was scientific rationality.
- Ding-Zhi-Xiao-Wan formula,
- Decompose prescription,
- Compatibility interaction,
- Aβ_25-35,
- PC12 cell,
- Cell apoptosis,
- Oxidative stress
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Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ25-35 Induced PC12 Cells

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通讯作者: 陈斌, bchen63@163.com

Study on Compatibility Mechanism of Ding-Zhi-Xiao-Wan Formula in Treating Alzheimer's Disease Based on Aβ_25-35 Induced PC12 Cells