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Citation: SUN Yan-Hong, WEI Jia, WANG Zhen-Xin, MENG Xian-Ying. Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(9): 1357-1362. doi: 10.11895/j.issn.0253-3820.181293 shu

Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells

1.
Department of Thyroid Surgery, the First Hospital of Jilin University, Changchun 130021, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 4 May 2018
Revised Date: 27 June 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 81571737, 21475126).

Two kinds of ssDNA-GNPs(PolyG₁-GNPs and PolyG₂-GNPs) were synthesized by modification of two different G-rich single-strand oligonucleotides(PolyG₁ and PolyG₂) onto 13-nm gold nanoparticles (GNP) surface through formation of the sulfur-gold covalent bond, respectively. The as-prepared PolyG₁-GNPs and PolyG₂-GNPs had reasonable colloidal stability in complex dispersing matrices. The effect of ssDNA sequence on the interaction between ssDNA-GNPs and cells was systematically investigated by UV-vis absorption spectroscopy, cellular transmission electronic microscopy and inductively coupled plasma mass spectrometry. The experimental results showed that both of the as-prepared PolyG₁-GNPs and PolyG₂-GNPs had low cytotoxicity and exhibited energy related endocytosis. In addition, the cellular internalization amount and dispersibility of ssDNA-GNPs were strongly affected by the sequences of immobilized ssDNA. PolyG₂ with G4 secondary structure could significantly increase the cellular internalization amount and intracellular stability of PolyG₂-GNPs.
- G-rich single-stranded oligonucleotides,
- Living cells,
- Gold nanoparticles,
- Interaction
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1.
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